胰蛋白酶在水解猪肠黏膜制备肝素中的应用研究

Implication of Trypsin in the Heparin Preparation from Hydrolyzed Pig Intestine Mucosa

本文利用胰蛋白酶水解猪小肠黏膜制备肝素,在单因素试验基础上,利用Box-Behnken设计,对水解条件进行了优化研究,并进行了验证试验。结果表明,胰蛋白酶酶解条件为:酶底物比0.70%,温度60℃,时间3 h,pH值8.5,盐浓度0.3 mol?L?1,料液比为1:7 (g:L);验证试验证明该水解条件是可靠的。

In this paper, the implication of Trypsin in the heparin preparation from hydrolyzed pig intestine mucosa studied. On the basis of single-factor tests, the hydrolysis conditions were optimized by the Box-Behnken design, and the validation experiments were performed. The results showed that the Trypsin hydrolysis conditions were temperature 60?C, time 3 h, material liquid ratio 1:7 (g:L), enzyme substrate ratio 0.70%, pH 8.5, salt concentration 0.3 mol?L?1, and the validation experiment confirmed that the hydrolysis condition was reliable.