龟甲胶的快速制备工艺及其抗氧化活性研究

The Rapid Production Method and Antioxidant Activity of Turtle-Shell Glue

以中华草龟为原料,探讨超高压技术对龟甲胶出胶率、提取时间、抗氧化活性的影响。采用300 MPa-15 min、300 MPa-30 min、500 MPa-15 min、500 MPa-30 min四种超高压条件对龟甲进行预处理制备龟甲胶,并测定其抗氧化活性。结果显示,与传统制备工艺相比,超高压技术可大大提高龟甲胶的生产效率,其中300 MPa-30 min超高压处理组的提取时间可缩短42%。此外,不同方法制备的龟甲胶皆具有抗氧化活性,四种超高压处理组对•OH的清除率均高于传统工艺组,最高达37.39%;500 MPa-15 min、300 MPa-15 min、500 MPa-30 min组对DPPH•的清除率高于传统工艺组,最高达76.76%;500 MPa-15 min、500 MPa-30 min组对ABTS+•的清除率高于传统工艺组,最高达97.33%。本研究可为龟甲胶的快速制备工艺提供理论依据。

In this study, Chinese turtles were served as the experimental material to analyze the effect of ultra-high pressure on the yield, extraction time and antioxidant activity of turtle-shell glue. The turtle-shell were pretreated by four kinds of ultra-high pressure treatments: 300 MPa-15 min, 300 MPa-30 min, 500 MPa-15 min and 500 MPa-30 min, which were made into turtle-shell glue afterwards. The scavenging activity between turtle-shell glue made by ultra-high pressure and the traditional method was detected as well. The results showed that, in comparison with the traditional method, ultra-high pressure could greatly improve the production efficiency, and the extraction time of 300 MPa-30 min group could be reduced by 42%. Additionally, the turtle-shell glue made by different methods all had antioxidant activity. The clearance rates of •OH of four ultra-high pressure groups were higher than that of the traditional group, with a maximum of 37.39%;the clearance rates of DPPH• of 500 MPa-15 min, 300 MPa-15 min, and 500 MPa-30 min groups were higher than that of the traditional group, with a maximum of 76.76%;the clearance rates of ABTS+• of 500 MPa-15 min and 500 MPa-30 min groups were higher than that of the traditional group, with a maximum of 97.33%. This study can provide a theoretical basis for the rapid production method of turtle-shell glue.