荧光分光光度法测定乳粉中维生素C含量的不确定度评定

Uncertainty Assessment of Vitamin C Content in Milk Powder by Fluorescence Spectrophotometry

为了提高实验室检测数据的准确性,本文依据GB5413.18-2010《婴幼儿和乳品中维生素C的测定》方法,分析乳品中维生素C测定过程中的不确定度来源,建立了不确定度评估数学模型,评定出各不确定度分量。结果表明,当乳粉样品中维生素C的含量为52.0 mg/100g,扩展不确定度为3.0 mg/100mg,重复性测量和校准曲线的拟合是不确定度的主要来源。

In order to improve the accuracy of laboratory test results, this article analyzed the sources of uncertainty in the determination of vitamin C in dairy products based on the GB5413.18-20210 method “Determination of Vitamin C in Infants and Dairy Products”, established a mathematical model for uncertainty evaluation, and evaluated each uncertainty component. The results indicated that when the content of vitamin C in milk powder samples was 52.0 mg/100g, the extended uncertainty was 3.0 mg/100mg. and the fit of repeatability measurement and calibration curve was the main source of uncertainty.