响应面法优化黔产大蝎子草水溶性多糖提取工艺研究

Optimization of Extraction Process of Water-Soluble Polysaccharides from Girardinia diversifolia in Guizhou by Response Surface Methodology

目的:本次试验是针对黔产大蝎子草的水溶性多糖提取工艺进行优化。方法:以浸泡时间、料液比和提取时间为考察因素,以黔产大蝎子草总多糖含量为指标,进行单因素试验并设计Box-Behnken响应面试验。结果:大蝎子草水溶性多糖的最优提取工艺为:浸泡时间53 min、料液比1:15 g/mL、提取时间90 min,在该条件下测出总多糖含量为10.60 mg/g。结论:本次通过响应面法对大蝎子草水溶性多糖提取优化结果表明,该优化方法稳定、重复性好、操作便捷、简单易行,也为大蝎子草质量标准的完善提供了参考。

Objective: This experiment aims to optimize the extraction process of water-soluble polysaccha-rides from Girardinia diversifolia in Guizhou. Methods: Taking soaking time, solid-liquid ratio and extraction time as investigation factors and total polysaccharide content of Girardinia diversifolia in Guizhou, single factor test was conducted and box-Behnken response surface test was designed. Results: The optimal extraction process of water-soluble polysaccharides from Girardinia diversifo-lia was: under the soaking conditions of 53 min, solid-liquid ratio of 1:15 g/mL and extraction time of 90 min, the total polysaccharide content was 10.60 mg/g. Conclusion: The optimization results of the extraction of water-soluble polysaccharides from Girardinia diversifolia by response surface methodology show that the optimization method is stable, reproducible, convenient to operate, simple and easy to operate;it also provides references for the study on the extraction technology of water-soluble polysaccharide from Girardinia diversifolia.