基于网络药理学和实验验证探究苗药弩药治疗膝骨性关节炎的作用机制

Based on Network Pharmacology and Experimental Validation, to Investigate the Mechanism of Action of Miao Medicine and Crossbow in the Treatment of Knee Osteoarthritis

目的:运用网络药理学及实验研究苗药弩药治疗膝骨性关节炎的潜在的作用机制。方法:通过TCMSP、CNKI、BATMAN-TCM等数据库筛选弩药方潜在活性成分及作用靶点。检索OMIM、Drug bank、TDD及Genecards数据库获取膝骨性关节炎潜在作用靶点,进一步获取弩药方治疗膝骨性关节炎的潜在靶点。采用String数据库结合Cytoscape3.9.2绘制PPI网络。利用Metascape数据库对药物-疾病交集靶点进行GO及KEGG富集分析。采用RT-qPCR检测关节软骨中caspase-9 mRNA的表达。结果:共筛选得到38个活性成分,疾病与潜在活性成分共有143个交集靶点,其中包括ALB、AKT1、VEGFA、JUN、STAT3、MMP9等核心靶点;涉及的主要通路包括肿瘤通路、PI3K-Akt信号通路、MAPK信号通路等,弩药可显著降低关节软骨中caspase-9 mRNA的表达。结论:进一步阐明苗医弩药治疗膝骨性关节炎的潜在作用靶点及通路,有利于了解其作用机制,为后续相关研究奠定了基础。

Objective: Network pharmacology and experiments were used to study the potential mechanism of action of Miao Medicine and crossbow in the treatment of knee osteoarthritis. Methods: TCMSP, CNKI and BATMAN-TCM were used to screen potential active components and target of crossbow prescription. Search the OMIM, Drug bank, TDD and Genecards databases for potential targets of osteoarthritis/osteodystrophy for the treatment of knee osteoarthritis. PPI networks are drawn using String database with Cytoscape 3.9.2. Using Metascape database, GO and KEGG enrichment analysis was performed for drug-disease cross-targets. Results: A total of 38 active components were screened and 143 targets were identified, including ALB, Akt1, VEGFA, Jun, STAT3 and MMP9 The main pathways involved include tumor pathway, PI3K-Akt signaling pathway, MAPK signaling pathway and so on. The expression of caspase-9 mRNA in articular cartilage was significantly decreased by crossbow. Conclusion: Further elucidation of the potential targets and pathways of Miao medicine crossbow medicine in the treatment of knee osteoarthritis/osteodystrophy is helpful to understand its mechanism of action and lays a foundation for the follow-up research.