摘要
目的:探讨As2O3对ACC-2细胞端粒酶hTERT mRNA表达的影响。方法:进行ACC-2细胞培养,将As2O3建立不同药物浓度梯度(0、1.0、2.0、4.0、8.0 μmol/L)分别作用于ACC-2细胞24 h,48 h,应用RT-PCR方法,检测As2O3作用前后,ACC-2细胞的hTERT mRNA表达水平。结果:RT-PCR结果显示,在未经药物作用、作为对照组的正常培养的ACC-2细胞中,hTERT mRNA呈现明显高表达;在As2O3作用于ACC-2细胞后24 h与48 h时,随As2O3药物浓度的增高(1.0 μmol/L、2.0 μmol/L、4.0 μmol/L、8.0 μmol/L),hTERT mRNA表达逐渐降低(P As2O3可下调ACC-2细胞的hTERT mRNA转录表达,来诱导ACC-2细胞凋亡。
AIM: To investigate the effect of As2O3 on the expression of telomerase reverse transcriptase mRNA in ACC-2 cells. METHODS: ACC-2 cells were cultured. The different drug concentration gradient (0, 1.0, 2.0, 4.0, 8.0 μmol/L) of As2O3 were applied to ACC-2 cells for 24 h and 48 h respectively. The hTERT mRNA expression of ACC-2 cells before and after As2O3’s induction was detected by RT-PCR. RESULTS: RT-PCR results showed that hTERT mRNA expression was significantly higher in the control group, while after As2O3 action to ACC-2 cell for 24 h and 48 h, with the increase of As2O3 concentration (1.0 μmol/L, 2.0 μmol/L, 4.0 μmol/L, 8.0 μmol/L), hTERT mRNA expression decreased gradually (P As2O3 can induce apoptosis of ACC-2 cells by downregulating hTERT mRNA transcript expression.
出处
《眼科学》
2016年第4期95-104,共10页
Hans Journal of Ophthalmology
基金
山东省自然科学基金资助项目(No. ZR2012HM062)。