双芍护睛散抗大鼠外伤性视神经损伤的视网膜神经纤维层观察

Observation of Retinal Nerve Fiber Layer in Rats with Traumatic Optic Nerve Injury Treated by Shuang Shao Hu Jing Powder

目的:观察双芍护睛散抗大鼠外伤性视神经损伤的视网膜神经纤维层变化。方法:将实验SD大鼠60只随机抽取15只为空白对照组,其余45只造模后随机分为模型对照组、生理盐水组及中药治疗组,每组15只。空白对照组及模型对照组造模后24小时后,生理盐水组及中药治疗组(双芍护睛散)用药4周后取左眼观察视网膜神经节细胞及神经纤维层形态与神经纤维层厚度的变化。结果:模型对照组的视网膜中大量RGC肿胀,神经纤维层明显水肿增厚,其厚度与空白对照组比较有显著性差异(p <0.05)。生理盐水组视网膜大量RGC呈空泡样变性、脱落,神经纤维层显著变薄;而中药治疗组视网膜少量RGC轻度肿胀,神经纤维层轻度变薄,其厚度与生理盐水组比较有显著性差异(p <0.05)。结论:以养血行血法组方的双芍护睛散具有一定抗视神经的损伤,促进视网膜神经节细胞及视神经纤维修复的作用而保护视神经。

Objective: To observe the change of retinal nerve fiber layer of rats with traumatic optic nerve ?njury that treated by Shuang Shao Hu Jing Powder. Methods: 15 rats were randomly selected from 60 SD rats as control group, and the remaining 45 model rats were randomly divided into model control group, saline group and TCM treatment group;each group had 15 rats. Retina of the blank control group and the model control group were peeled after 24 hours, while that of the normal saline group and TCM treatment group (Shuang shao hu jing powder) were peeled after 4 weeks of treatment. Changes of retinal ganglion cells and nerve fiber layer morphology and thickness were observed. Results: In the model control group, there was a large amount of RGC in the retina, and the nerve fiber layer was significantly thickened. The thickness of the nerve fiber layer was significantly different from that of the control group (P < 0.05). A large number of RGC in the normal saline group showed vacuolar degeneration, shedding, and the nerve fiber layer became thinner;while in the treatment group, there was a slight swelling of RGC in the retina and a slight thinning of the nerve fiber layer, and there was significant difference between the treatment group and the saline group (P < 0.05). Conclusion: Shuang Shao Hu Jing Powder, with a combined function of nourishing blood to activate blood, showed certain effects of anti-optic-nerve injury, promoting retinal ganglion cells’ development and optic nerve fiber recovery, thus, protecting the optic nerve.