夏侧金盏花中化学成分的分离鉴定及活性研究

Isolation, Identification and Bioacitivity Study of Chemical Components in Adonis aestivalis L.

夏侧金盏花(Adonis aestivalis L.)是一种毛茛目,毛茛科的一年生草本植物,利用有机溶剂进行提取得到粗提物,所得粗提物用正相硅胶、反相硅胶、SephadexLH-20凝胶与制备薄层色谱(pTLC)等进行分离得到7个单体化合物,通过核磁共振波谱、质谱等鉴定得出化合物的结构,其中化合物1、2、3、4都是黄酮类化合物,化合物5、6、7为甾体类化合物,对所得到的化合物进行相关性质测试,并对所得到的化合物进行了DPPH活性测试,黄酮类化合物的抗氧化活性较强,为夏侧金盏花的种植使用提供了科学的依据。

Adonis aestivalis L.is a kind of annual herb of the genus Ranunculaceae.The crude extract was ex-tracted with organic solvent and 7 compounds were obtained from it.These compounds were iso-lated by a combination of usage of silica gel,Sephadex LH-20 and preparative of thin layer chro-matography(pTLC).The structures of the compounds were identified by nuclear magnetic reso-nance spectroscopy,mass spectrometry,etc.Compounds 1,2,3,and 4 were all flavonoids,and compounds 5,6 and 7 were steroids.The compounds were tested;DPPH activity and the activity of the flavonoid analogues were potent,which provided a scientific basis for the planting of the summer marigold flower.