HA-DAP水凝胶的制备及其对内皮细胞生物学行为的影响

Preparation and Its Influence of HA-DAP Hydrogel on the Biological Behavior of the Endothelial Cells

制备不同多巴胺反应浓度的透明质酸水凝胶,研究其对内皮细胞粘附行为、增殖能力与活性的影响。通过紫外可见光谱(UVVis)检测透明质酸与多巴胺反应后是否有酚羟基特征吸收峰,通过扫描电镜观察水凝胶的断面形貌,通过溶胀平衡法测试水凝胶的溶胀率,通过荧光染色法和CCK-8细胞增殖活性检测手段对HA-DAP (HA:透明质酸,DAP:多巴胺)水凝胶表面内皮细胞生长行为进行评价。结果显示,成功制备出不同多巴胺反应浓度的透明质酸水凝胶,透明质酸水凝胶的孔洞直径在30~120 μm,随着多巴胺浓度增加,凝胶孔洞直径呈减小趋势。同时,多巴胺反应浓度为3 mg/ml的透明质酸水凝胶的细胞相容性(内皮细胞粘附,内皮细胞静态培养1天、3天数量、形态和活性)优于其他样品。

The ideal biomaterials surface for vascular contacting should be endothelialization ability. To im-prove the inducing endothelialization ability of material surface, this research focuses on a new technique of hyaluronic acid-dopamine (HA-PDA) hydrogel. Several bio-functional HA-PAD hydrogel made of dopamine and hyaluronic acid in different ratios were fabricated. The catechol was determined by ultraviolet-visible (UVVis) spectroscopy at 280 nm. The cross-sectional morphology of the hydrogels was observed by scanning electron microscopy (SEM), and the swelling rate of the hydrogels was measured by the swelling equilibrium method. The morphology and quantity of cells on the surface were detected by rhodamine staining and CCK-8. The results showed that the HA-DAP hydrogel made of dopamine and hyaluronic acid in different ratios was successfully constructed. The pore size of HA-DAP hydrogel was 30 - 120 μm, and the pore size of hydrogel decreased with the increase of dopamine concentration. HA-DAP3 (hyaluronic acid: 10 mg/ml, dopamine: 3 mg/ml) provided favorable cell compatibility according to the endothelial cells adhesion, proliferation and its biological activity based the CCK-8 measurements. We hope that HA-DAP hydrogel can provide more helpful exploration and application for promoting endothelialization on cardiovascular stents.