基于乙酰胆碱酯酶的一次性可抛纸基酶传感器应用于杀螟硫磷的检测研究

Study on the Application of Acetylcholinesterase-Based Disposable Paper-Based Sensor to the Detection of Fenitrothion

本研究旨在研发了一种应用于有机磷农药快速、准确、一次性可抛的传感装置。用碳胶/氧化铟锡玻璃(ITO)作为工作电极,碳和银作为对电极和参比电极构成基于三电极系统。纳米金铂–石墨烯材料修饰在工作电极表面用来固定乙酰胆碱酯酶(AChE)和加速电子传递速度。以氯化乙酰胆碱为底物,通过AChE酶催化反应检测底物杀螟硫磷。结果表明,使用差分脉冲伏安法检测杀螟硫磷,其浓度从1.0 ×10−7到1.5 ×10−5 mol/L范围内,氧化峰电流的降低程度与杀螟硫磷浓度的对数成正比,检测限低至5.4 ×10−8 mol/L。该酶传感器有希望成为快速检测有机磷农药的新方法。

Thepurpose is to develop a rapid, accurate, disposable sensing device applied toorganophosphrus pesticides (OPs). Using carbon glue/indium tin oxide glass(ITO) as working electrode, carbon glue and silver glue as counter andreference electrode, a three-electrode system was formed. Gold/ platinum-graphene nanocomposites were modified toimmobilize acetylcholinesterase (AChE) and enhance electron transfer rate.Acetylcholine chloride was used as substrate to detect fenitrothion by AChEenzyme catalyzed reaction. The results showed that the reduction of oxidationpeak current was directly proportional to the logarithm of the concentration offenitrothion in the range of 1.0 ×10−7 to 1.5 ×10−5 mol/Lby differential pulse voltammetry (DPV). The detection limit was as low as 5.4 ×10−8 mol/L. The enzyme sensor is expected to be a new method forrapid and economical detection of OPs.