虫纹鳕鲈<i>Maccullochella peelii</i>染色体核型分析

Karyotype of the <i>Maccullochella peelii</i>

采用PHA体内直接注射法制备了虫纹鳕鲈(Maccullochella peelii, Mitchell)染色体标本,经空气干燥法,Giemsa染色后,观察虫纹鳕鲈的染色体核型和特征,结果表明,虫纹鳕鲈染色体数目为48条,2n = 48所占的观察分裂相中的比例为82%;核型公式为:2n = 2sm + 10st + 36t,染色体臂数(NF)为50,其染色体相对长度范围为(5.40 &#177;0.02)^(2.56 &#177;0.01),未发现与性别相关的异型染色体。通过比较分析,虫纹鳕鲈属于高位类类群。本研究为麦鳕鲈属鱼类的细胞遗传学研究提供了重要资料,并为其种子资源的保护及人工繁育育种等奠定基础。

Metaphase of kidney cells was collected to study the karyotype of Murray Cod by in vivo injection of PHA and colchicine, chromosome samples were obtained by using the olchicine-air drying technique, and Giemsa staining. The results showed that the diploid chromosome number of Murray Cod was 48, and 82 spreads showed 2n = 48 chromosomes, which represented 82% of the observed metaphase. The karyotypic formulae are 2n = 2sm + 10st + 36t and NF = 50. The relative length of chromosome was in the range of (5.40 &#177;0.02) - ( 2.56 &#177;0.01), and the sex chromosome were not found in this species. The comparison of karyotyaes suggested that M. peelii might belong to an advanced and specific evolutionary group. Our study will promote the understanding of the cytogenetic research of the genus Maccullochella, and lay the foundation for the protection of germplasm resources and artificial breeding.