2B-RAD测序技术在水产动物中的研究进展

The Research Progress of 2B-RAD Sequencing Technology in Aquatic Animals

后基因组时代背景下测序技术的不断提升推动了水产中模式生物和非模式生物基因组的研究,其中基于IIB型限制性内切酶进行大规模SNP标记开发及分型的2B-RAD测序技术广泛应用在水产动物的群体进化和遗传结构功能分析。本文综述了2B-RAD测序技术发展历程及其在水产动物种群遗传学分析、进化分析、重要性状关联等研究领域的应用,以期为水产动物分子辅助育种及种质资源保护的研究工作提供参考依据。

In post-genomic era, a constant improvement in sequencing technology enhanced the study on genome in aquatic model and non-model organisms. 2B-RAD, a sequencing technology for developing large-scale molecular markers and genotyping based on type IIB restriction enzymes, is widely used in the population evolution and genetic structure function analysis of aquatic animals. This paper summarizes the basic work principle and application of 2B-RAD in the research fields of aquatic population genetic analysis, evolutionary analysis, importance traits and so on to provide a referential basis for the research of molecular marker-assisted breeding and germplasm conservation.