基于大豆磷脂的氮磷掺杂碳量子点测定环境水样中的高锰酸盐

Determination of Permanganate in Environmental Water Samples Based on Nitrogen and Phosphorus Doped Carbon Dots Derived from Soybean Phospholipids

以大豆磷脂为碳源,采用一步水热法成功制备了氮、磷共掺杂碳量子点(N, P-CDs)。采用KBH4对所制备的N,P-CDs进行还原处理后,得到还原态氮磷掺杂碳量子点(r-N, P-CDs)。r-N,P-CDs具有蓝色荧光(Ex 340 nm, Em 425 nm),荧光量子产率为10%。通过研究发现,在酸性条件下,高锰酸盐( )通过氧化作用而对r-N,P-CDs具有明显的猝灭作用,基于该荧光猝灭效应,建立了MnO4-荧光传感分析方法。该方法检出限(3σ)为10.0 ng∙mL−1,相对标准偏差(RSD)为1.2% (c = 1.0 μg∙mL−1, n = 15),其中线性范围分别为0.1~2 μg∙mL−1和5~50 μg∙mL−1。所建立的分析方法成功应用于环境水样中MnO4-的荧光测定,加标回收实验的回收率在94.0%~113%的范围。

Nitrogen and phosphorus co-doped carbon dots (N, P-CDs) were successfully fabricated by a one-step hydrothermal method using soybean phospholipids as the carbon source. The reduced N, P-CDs (r-N, P-CDs) were obtained after the reduction by KBH4. The r-N, P-CDs possess blue fluorescence (Ex 340 nm, Em 425 nm) with a fluorescence quantum yield of 10%. It was found that the fluorescence of r-N, P-CDs could be quantitatively quenched by permanganate (MnO4-) through oxidation reaction under acidic media. Based on this fluorescence quenching effect, a new fluorescence sensing method for was developed. The detection limit (3σ) of the developed method is 10.0 ng∙mL−1, the relative standard deviation (RSD) is 1.2% (c = 1.0 μg∙mL−1, n = 15), and the linear range is from 0.1 to 2 μg∙mL−1 and from 5 to 50 μg∙mL−1, respectively. The method was successfully applied to the determination of MnO4- in environmental water samples with recoveries in the recoveries range of 94.0%~113%.