白纹伊蚊体内人血源DNA的STR分型检测

Short Tandem Repeat Typing of Human Blood-Derived DNA in Aedes albopictus

探讨白纹伊蚊吸血后不同时间段提取人类DNA的可能性,并探究用于个人识别的可行性。运用Chelex-100法和硅膜纯化法提取白纹伊蚊吸食人血后体内消化物DNA,测定OD值,对DNA进行定量分析,并采用PCR-STR分型技术对D3S1358等21个基因座进行分型。采用Chelex-100法提取DNA可以在白纹伊蚊吸血后60小时内获得完整的STR分型图谱,吸血后72小时扩增结果未成功;采用硅膜纯化法,成功获得吸血后72小时完整图谱,STR分型结果与志愿者完全相同。白纹伊蚊体内提取的人血源DNA可用于DNA分型及个人识别鉴定,是有价值的法医学证据。

To explore the possibility of extracting human DNA at different times after blood feeding of Aedes albopictus and explore the feasibility of individual identification. Chelex-100 method and silicon membrane purification method were used to extract the digested DNA of Aedes albopictus after sucking human blood. OD value was determined and the DNA was quantitatively analyzed, and 21 loci, including D3S1358, were typed by PCR-STR typing technology. The complete STR genotyping profiles of Aedes albopictus were obtained within 60 hours after blood feeding by using Chelex-100 method, but the amplification was not successful at 72 hours after blood feeding. The complete profiles were obtained at 72 hours after blood feeding by silicon membrane purification method, and the STR typing results were identical to those of volunteers. Human blood DNA extracted from Aedes albopictus can be used for DNA typing and individual identification, which is valuable forensic evidence.