响应面法优化超声辅助竹茹总黄酮提取工艺研究

Optimizing of Ultrasonic-Assisted Extraction Technology of Total Flavonoids from Bambusae caulis in Taenias Based on Response Surface Methodology

目的:研究超声辅助提取竹茹总黄酮的最佳工艺条件。方法:在单因素试验基础上,选取乙醇浓度、料液比、超声功率及超声时间为影响因素,采用中心组合实验设计,以竹茹总黄酮提取率为响应值进行响应面分析。结果最佳工艺条件为:乙醇浓度67%、料液比1:25、超声功率330 W、超声时间26 min,竹茹总黄酮得率为3.557%。结论:该工艺提取率高、操作简便,可为竹茹总黄酮的提取提供参考。

Objective: To optimize of ultrasonic-assisted extraction technology of total flavonoids from Bambusae caulis in taenias based on response surface methodology. Method: On the basis of single-factor experiment, four parameters including ethanol concentration, solid-liquid ratio, ultrasonic power and ultrasonic time were selected as influencing factors and the optimum conditions for total flavonoids extract were obtained through Box-Behnken center united experiment designed by response surface methodology. Results: The optimum conditions were as follows;the ethanol concentration was 67%;the solid-liquid ratio was 1:25;the ultrasonic power was 330 W and the ultrasonic time was 26 min. Under the conditions, the extraction rate of total flavonoids could be reached to 3.557%. Conclusion: This method is simple, rapid and reliable which could provide reference for the producing of total flavonoids from Bambusae caulis in taenias.