野巴子粗多糖的提取及生物活性测定

Extraction and Bioactivity Determination of Polysaccharide from Elsholtzia rugulosa Hemsl.

采用超声波辅助水提醇沉法从野巴子叶中提取野巴子粗多糖(YBZc),野巴子多糖的提取率为5.63%,硫酸–咔唑法测得其糖醛酸含量为32.2%,凯氏定氮法测得蛋白质含量为2.98%。气相色谱分析结果表明YBZc的单糖组成为Rha、Ara、Man、Glc、Gal,相对摩尔比为1.000:0.793:0.440:0.557:0.994。YBZc的活性实验表明,在浓度达到3.2 mg/mL时,YBZc对?OH、DPPH?的清除率最大分别为50.14%、97.37%,且在一定的范围内存在量效关系;在浓度达到1.0 mg/mL时,YBZc对α-葡萄糖苷酶抑制效果最佳,为90.28%,且在一定的范围内存在量效关系。表明YBZc具有一定的清除?OH和DPPH?的活性以及降血糖效果。YBZc能减弱STS介导的乳鼠海马神经元损伤,具有对海马神经细胞的保护作用。

Ultrasound-assisted water extraction and alcohol precipitation method was used to extract the polysaccharide from Elsholtzia rugulosa Hemsl.(YBZc),the extraction rate is 5.63%.The uronic acid content was determined by the sulfuric acid-carbazole method to be 32.2%,and the protein content measured by the Kjeldahl method was 2.98%.The results of gas chromatography showed that the monosaccharide composition of YBZc was Rha,Ara,Man,Glc,Gal,and the relative molar ratio was 1.000:0.793:0.440:0.557:0.994.When the concentration reached 3.2 mg/mL,the maximum clearance rate of YBZc to?OH and DPPH?was 50.14%and 97.37%,respectively,and there was a dose-effect relationship in a certain range;when the concentration reached 1 mg/mL,YBZc had the best inhibitory effect onα-glucosidase,which was 90.28%,and there is a dose-effect relationship within a certain range.It indicates that YBZc has certain activities of clearing?OH and DPPH?and hypoglycemic effects.YBZc can attenuate STS-mediated hippocampal neuronal damage in rats and has protective effects on hippocampal neurons.