HPLC测定迷迭香中齐墩果酸、熊果酸的含量

Determination of Oleanolic Acid and Ursolic Acid in Rosmarinus officinalis by HPLC

利用高效液相色谱法,同时检测迷迭香中齐墩果酸、熊果酸的含量,用安捷伦色谱分析柱(250 mm ×4.6 mm, 5 μm),氨水(0.1%):乙腈(磷酸调节pH = 7.3) (35:65)为流动相,流速1.2 mL/min,柱温25℃,测量波长为206 nm。结果:1) 齐墩果酸在13.7 min左右出峰,在194~970 μg/mL内的线性关系良好,R值为0.9992,平均回收率为98.83%,RSD为1.96%;2) 熊果酸在15.0 min左右出峰,在52.75~1055 μg/mL内线性相互作用良好,R值为0.9994,平均回收率为99.36%,RSD为1.93%。两者分离度为1.81,理论塔板数大于5000。结论:该方法实现了迷迭香中齐墩果酸、熊果酸的分离及含量检测,且方法准确、可靠。经检测,迷迭香样品含有齐墩果酸约1.54%、熊果酸约2.97%,开发了丰富的齐墩果酸、熊果酸植物资源。

The contents of oleanolic acid and ursolic acid in Rosmarinus officinalis were simultaneously determined by high-performance liquid chromatography (HPLC), and Agilent chromatographic column (250 mm ×4.6 mm, 5 μm), acetonitrile: ammonia (0.1%) (adjusted PH = 7.3 by phosphoric acid) (65:35) as the mobile phase, flow rate of 1.2 mL/min, column temperature of 25℃, measurement wavelength of 206 nm. Results: 1) oleanolic acid peaked at about 13.7 min, ranging from 194 to 970 μg/mL. The linear relationship was good in the range of 0.9992, the average recovery was 98.83%, and the RSD was 1.96%;2) Ursolic acid peaked at about 15.0 min, ranging from 52.75 to 1055 μg/mL. The linear interaction was good in the range of 0.9994, the average recovery was 99.36%, and the RSD was 1.93%. The separation of the two is 1.81 and the number of theoretical trays is more than 5000. Conclusion: the method can be used for the separation and determination of oleanolic acid and ursolic acid in Rosmarinus officinalis, and the method is accurate and reliable. After testing, the sample of Rosmarinus officinalis contains about 1.54% oleanolic acid and about 2.97% ursolic acid. It has developed rich plant resources of oleanolic acid and ursolic acid.