基于网络药理学探讨山茱萸治疗心源性休克的作用机制研究

Study on the Mechanism of the Action of Cornus officinalis in Treating Cardiogenic Shock Based on Network Pharmacology

目的:探究山茱萸干预心源性休克的潜在机制。方法:从TCMSP数据库获取山茱萸的有效成分和潜在靶点。收集GeneCards、OMIM、Drugbank和PharmGkb等数据库中的心源性休克相关基因。对两者取交集后找出发挥作用的化合成分和潜在靶点。对潜在靶点进行PPI、GO、KEGG分析,探究山茱萸干预心源性休克的作用机制。结果:通过TCMSP共找出山茱萸中的20个化学成分,54个靶点。综合所有疾病数据库共获得492个心源性休克相关基因。两者取交集后得到15个山茱萸干预心源性休克潜在靶点。PPI结果显示,PTGS2、ADRB2、DRD1、ADRA1A、ADRA1B、CASP3之间有较强的相互作用关系。GO结果表明,BP与腺苷酸环化酶激活肾上腺素能受体信号通路、肾上腺素能受体信号通路、管径调节的调节有关。CC与膜筏、膜微域、突触前膜的内在成分有关。MF参与G蛋白偶联胺受体活性、儿茶酚胺结合、参与凋亡信号通路的半胱氨酸型肽酶活性有关。KEGG结果显示,交集基因与钙信号通路、cGMP-PKG信号通路、唾液分泌、心肌细胞肾上腺素能信号通路、IL-17信号通路、组氨酸代谢、TNF信号通路有关。结论:山茱萸可能通过调节PTGS2、ADRB2、DRD1、ADRA1A等靶点进而干预心源性休克患者的预后。

Objective: To explore the potential mechanism of Cornus officinalis intervention in cardiogenic shock. Methods: The active components and potential targets of Cornus officinalis were obtained from the TCMSP database. Collect cardiogenic shock-related genes from GeneCards, OMIM, Drug-bank and PharmGkb databases. Find out the active compounds and potential targets after the intersection of the two. PPI, GO and KEGG were analyzed for potential targets to explore the mechanism of the action of Cornus officinalis intervention in cardiogenic shock. Results: A total of 20 chemical constituents and 54 targets were identified from Cornus officinalis by TCMSP. A total of 492 cardiogenic shock-related genes were obtained from all disease databases. Fifteen potential targets of Cornus officinalis for cardiogenic shock intervention were obtained after the intersection of the two. PPI results showed that PTGS2, ADRB2, DRD1, ADRA1A, ADRA1B, CASP3 had a strong interaction relationship. GO results showed that BP was related to the activation of adrenergic receptor signal pathway by adenylate cyclase, the regulation of adrenergic receptor signal pathway and the regulation of pipe diameter. CC is related to the internal components of membrane raft, membrane microdomain and presynaptic membrane. MF is involved in G protein-coupled amine receptor activity, catecholamine binding and cysteine peptidase activity involved in the apoptosis signal pathway. KEGG results showed that the cross genes were related to calcium signaling pathway, cGMP-PKG signaling pathway, salivary secretion, adrenergic signaling pathway, IL-17 signaling pathway, histidine metabolism and TNF signaling pathway. Conclusion: Cornus officinalis may interfere with the prognosis of patients with cardiogenic shock by regulating PTGS2, ADRB2, DRD1, ADRA1A and other targets.