不同检验体系中白色念珠菌检查法的比较研究

Comparative Study of Candida albicans Test Methods in Different Test Systems

目的:比较国内外不同检验领域中现行版本的白色念珠菌检查方法及标准的差异,为我国化妆品相关微生物标准的修订和完善提供参考。方法:比较中国药典、我国出入境检验检疫行业标准化妆品、出口食品中白色念珠菌检测方法(包括样品前处理、增菌、分离、鉴定等)的差异。结果与结论:不同检验体系中的微生物标准中虽然培养基体系不同,但同一种微生物特性不变,在鉴定时存在参考借鉴的可能。建议相互取长补短,从实验具体操作和实验思路拓展等方面提出可行性的参考方案,为《化妆品安全技术规范》中白色念珠菌检查法的修订提供参考,提高我国化妆品微生物控制水平,保障广大人民群众的用妆安全。

Objective: To compare the current methods and standards of Candida albicans in different testing fields at home and abroad, and to provide reference for the revision and improvement of the standards of cosmetics related microorganisms in China. Methods: The differences of detection methods of Candida albicans in cosmetics and export food were compared between Chinese pharmacopoeia and Chinese entry-exit inspection and quarantine industry standards, including sample pretreatment, bacteria enhancement, isolation and identification. Results and Conclusion: Although the medium system is different, the characteristics of the same microorganism are the same, and there is a possibility of reference in the identification. It is suggested to learn from each other’s strengths and weaknesses, and put forward a feasible reference scheme from the aspects of the specific operation of the experiment and the expansion of the experimental ideas, so as to provide a reference for the revision of the Candida albicans inspection method in the Technical Specification for Cosmetic Safety, improve the level of microbial control of cosmetics in China, and ensure the safety of the people.