片仔癀对乳腺癌细胞凋亡的影响

Apoptosis Effect of Pien Tze Huang on Breast Cancer Cells

目的:探讨片仔癀(Pien Tze Huang)对乳腺癌细胞(MDA-MB-231和MCF-7)凋亡的影响。方法:采用噻唑蓝/MTT测定细胞的存活率;使用流式细胞仪测定AnnexinV-FITC/PI细胞凋亡及细胞周期检测;应用Western Blot测定凋亡相关蛋白Bax和Bcl-2。结果:片仔癀对MDA-MB-231的细胞存活率呈时间和浓度相关性的显著性影响效果(p 【0.05)。AnnexinV-FITC/PI和细胞周期结果均表明,片仔癀可显著性地引起乳腺癌细胞的凋亡(p 【0.05)。同时,片仔癀可显著性地减少乳腺癌细胞中的抗凋亡蛋白Bcl-2的表达;相反地,片仔癀可显著性地增加乳腺癌细胞中的促进凋亡蛋白Bax的表达。结论:片仔癀可引起乳腺癌细胞凋亡,其在乳腺癌的治疗价值需要进一步探究。

Objective: To investigate the apoptosis effect of Pien Tze Huang on breast cancer cells. Methods: MTT assay was performed to determine the cell viability of breast cancer cells. Further, both An-nexinV-FITC/PI expression and cell cycle of breast cancer cells were evaluated by flow cytometry. Moreover, apoptosis-related proteins, including Bcl-2 and Bax, were analyzed to evaluate. Results: MTT assay found that Pien Tze Huang induced apoptosis of breast cancer cells by a dose- and time-dependent manner. Both results of AnnexinV-FITC/PI and cell cycle further indicated that Pien Tze Huang resulted in significant apoptosis effect of breast cancer cells (p