茯苓和首乌龙眼口服液对老年痴呆症(AD)小鼠模型治疗效果的实验研究

Experimental Study on the Effect of Tuckahoe and Shouwulongyan Oral Liquid on Alzheimer’s Disease (AD) Mouse Model

目的:研究中药茯苓水提液对老年痴呆症(AD)小鼠模型治疗效果并与复方何首乌口服液对比铰。方法:首先建立AD模型。用17.78%氯化铝 + 1.2% D-半乳糖 + 0.9%亚硝酸钠混合给小鼠腹腔注射造模二个月,然后用两种中药分别灌胃治疗二个实验组,20天结束。测定实验前、中、后的血红蛋白,用水迷宫试验测定小鼠记忆力前、中、后的变化。结束,取血测定血清生化指标,处死小鼠,取大脑,用生理盐水制成10%脑匀浆,测定谷胱甘肽过氧化物酶(GSH-px)、胆碱酯酶(AchE)、胆碱乙酰基转移酶(chAT)等。结果:正常对照组、模型对照组、实验(治疗1,茯苓组) 1组、实验(治疗2,桂元复方组) 2组依次排列,脑自由基,19.12 ±3.03▲▲,5.48 ±5.36,16.64 ±2.65▲▲,14.95 ±1.87▲▲★ (清除率%),F = 17.750,P = 0.000,差异有显著性意义;(GSH-px);脑(GSH-px),45.20 ±4.42▲▲★、43.56 ±8.73▲▲★、51.61 ±3.46、54.14 ±4.51 (活力/ml) F = 6.065,P = 0.004,差异有显著性意义;脑(chAT)活力,53.94 ±24.09★▲▲、15.15 ±5.91▲▲、62.15 ±29.94★★▲、94.85 ±33.12★★ (U/g组织湿重),F = 9.544,P = 0.000,★P 0.05,差异无显著性意义;水迷宫试验游水时间比较,正常对照组前、中、后差异无统计学意义;其余三组,造模后均明显延长,治疗后,模型对照组延长,实验1组、实验2组有一定程度缩短;血红蛋白(Hb)含量,正常对照组、模型对照组无明显变化,治疗两组均有升高。治疗后血清甘油三酯、尿素氮、总蛋白、(ALT/GPT)酶活力,各组间均有一定程度变化。结论:造模各组记忆力均明显下降,治疗后明显升高,疗效显著。

Objective: To study the therapeutic effect of traditional Chinese medicine Fuling water extract on Alzheimer’s disease (AD) mouse model and compare it with compound Polygonum multiflorum oral liquid. Methods: AD model was first established. The mice were intraperitoneally injected with 17.78% aluminum chloride + 1.2% D-galactose + 0.9% sodium nitrite for two months, and then the two experimental groups were treated by gavage with two kinds of traditional Chinese medicine respectively, ending with 20 days. Hemoglobin levels were measured before, during, and after the experiment, and memory changes were measured before, during, and after the water maze test. In the end, blood was taken to measure serum biochemical indexes, mice were sacrificed, brains were taken, 10% brain homogenate was prepared with normal saline;glutathione peroxidase (GSH-PX), cholinesterase (AchE), choline acetyltransferase (chAT) and so on were measured. Results: Normal control group, model control group, experimental group (treatment 1, Poria cocos group) 1, experimental group (treatment 2, Guiyuan compound group) 2 were arranged in order;brain free radical , 19.12 ±3.03▲▲, 5.48 ±5.36, 16.64 ±2.65▲▲, 14.95 ±1.87▲▲★ (clearance rate %), F = 17.750, P = 0.000, the difference was significant;(gsh-px);brain (GSH-PX), 45.20 ±4.42▲▲★, 43.56 ±8.73▲▲★, 51.61 ±3.46, 54.14 ±4.51 (vitality/mL) F = 6.065, P = 0.004. Brain (chAT), 53.94 ±24.09★▲▲, 15.15 ±5.91▲▲, 62.15 ±29.94★★▲, 94.85 ±33.12★★ (U/g tissue wet weight), F = 9.544, P = 0.000, ★P 0.05, with no significant difference. Compared with the water maze test, there was no statistically significant difference between the control group before, during and after the test. In the other three groups, it was significantly prolonged after modeling. After treatment, it was prolonged in the model control group and shortened to a certain extent in experimental group 1 and experimental group 2. The hemoglobin (Hb) content in the normal control group and the model control group showed no significant changes, and it increased in both treatment groups. After treatment, serum triglyceride, urea nitrogen, total protein and enzyme activity (ALT/GPT) were changed to some extent between each group. Conclusion: Memory decreased significantly in each group, and increased significantly after treatment.