缩泉益肾方对高糖诱导的HK-2细胞炎症因子影响的研究

Study on the Effect of Suoquan Yishen Formula on HK-2 Cell Inflammatory Factors Induced by High Sugar

目的:观察缩泉益肾方对高糖(HG)诱导的HK-2细胞炎症因子的影响。方法:将HK-2细胞分成N组、HG组、5% SQYSF + HG组、10% SQYSF + HG组、20% SQYSF + HG组、Cana + HG组。经过细胞干预24 h后,使用CCK8法检测细胞存活比率,流逝细胞仪检测细胞凋亡比率,ELISA法检测细胞中TNF-α、IL-1β及IL-6炎症因子水平。使用GraphPad Prism 9.5对实验数据进行统计处理。结果:与N组相比,HG组细胞存活率明显降低、细胞凋亡率和炎性因子浓度显著增加(P < 0.05);且HG组与5% SQYSF + HG组(简称SL组)、10% SQYSF + HG组(简称SM组)、20% SQYSF + HG组(简称SH组)及Cana + HG组(简称Cana组)相比,差异具有统计学意义(P < 0.05)。与HG组比较,Cana组、SL组、SM组、SH组HK-2细胞存活率明显升高(P < 0.05)。对于TNF-α、IL-6、IL-1β炎症因子水平的影响,HG组与SL组、SM组、SH组及Cana组差异明显(P < 0.05)。结论:缩泉益肾方可降低HG诱导下HK-2细胞炎症因子水平。

Objective: To observe the effect of Suoquan Yishen Formula on HK-2 cell inflammatory factors induced by high glucose (HG). Methods: HK-2 cells were divided into N group, HG group, 5% SQYSF + HG group, 10% SQYSF + HG group, 20% SQYSF + HG group and Cana + HG group. After 24 h of cell intervention, cell survival ratio was detected by CCK8 assay, cell apoptosis ratio was detected by elaptometry, and levels of TNF-α, IL-1β and IL-6 inflammatory factors were detected by ELISA. Statistical processing of experimental data was performed using GraphPad Prism 9.5. Results: Compared with N group, the survival rate of HG group was significantly decreased, the apoptosis rate and inflammatory factor concentration were significantly increased (P < 0.05). The differences between HG group and 5% SQYSF + HG group (SL group), 10% SQYSF + HG group (SM group), 20% SQYSF + HG group (SH group) and Cana + HG group (Cana group) were statistically significant (P < 0.05). Compared with HG group, the survival rate of HK-2 cells in Cana group, SL group, SM group and SH group was significantly increased (P < 0.05). There were significant differences between HG group and SL group, SM group, SH group and Cana group on the levels of TNF-α, IL-6 and IL-1β (P < 0.05). Conclusion: Suoquan Yishen Formula can reduce the level of HK-2 cell inflammatory factors induced by HG.