艾纳香纳米乳的制备及质量评价

Preparation and Quality Evaluation of Blumea balsamifera Nanoemulsion

目的:优化艾纳香纳米乳的制备工艺,并对其进行质量评价。方法:以粒径和PDI为指标,进行单因素考察和正交试验优化处方并制备艾纳香纳米乳,考察其外观形态,结构类型,pH值,粒径,多分散系数以及稳定性。结果:艾纳香纳米乳的优化处方为:吐温-80 4.00 g与正丁醇1.00 g,艾纳香油为油相0.21 g,艾纳香水提物溶液5.21 mL。该处方制备出的艾纳香纳米乳为浅棕色澄清透明液体,结构为O/W型,pH值为6.69左右,平均粒径为11.37 nm,在微观状态下呈圆球形且分布均匀。离心后未出现破乳、分层等现象,具有较好的稀释稳定性,并且其在4℃环境中放置28天是相对稳定的。气相色谱法测得艾纳香纳米乳中左旋龙脑的含量为0.8487 mg/g。结论:所制备的艾纳香纳米乳外观透明,质量良好,稳定性好。

Objective: To optimize the preparation process of Blumea balsamifera nanoemulsion and evaluate its quality. Methods: Single factor investigation and orthogonal test were used to optimize the prescription and prepare Blumea balsamifera nanoemulsion with particle size and PDI as indexes. The appearance, structure type, pH value, particle size, polydispersity coefficient and stability were investigated. Results: The optimized formulation of Blumea balsamifera nanoemulsion was as follows: Tween-80 4.00 g and n-butanol 1.00 g, Blumea balsamifera oil as oil phase 0.21 g, Blumea balsamifera water extract solution 5.21 mL. The Blumea balsamifera nanoemulsion prepared by this prescription is a light brown clear transparent liquid, the structure is O/W type, the pH value is about 6.69, the average particle size is 11.37 nm, and it is spherical and evenly distributed in the microscopic state. After centrifugation, there was no demulsification and stratification, and it had good dilution stability, and it was relatively stable at 4˚C for 28 days. The content of L-borneol in Blumea balsamifera nanoemulsion was 0.8487 mg/g by gas chromatography. Conclusion: The prepared Blumea balsamifera nanoemulsion has transparent appearance, good quality and good stability.