基于生物信息学、网络拓扑策略探究针刺干预帕金森病的中枢机制

Exploring the Central Nervous System Mechanism of Acupuncture for Parkinson’s Disease Based on Bioinformatics and Network Topology Strategies

目的:运用文本挖掘、生物信息学、网络拓扑学等方法,探究针刺治疗帕金森病(PD)的多靶点中枢机制。方法:检索文献发现针刺后产生的活性化合物,通过STITCH和SwissTargetPrediction数据库预测潜在作用靶点。利用人类基因数据库、在线人类孟德尔遗传数据库和全球蛋白资源数据库筛选出PD相关的疾病基因靶点。运用Cytoscape3.10.1软件构建共有靶点蛋白质–蛋白质互作网络图,通过DAVID数据库对共有靶点分别进行基因本体富集分析和京都基因组百科全书富集分析并可视化。结果:共鉴定了114个与针刺治疗帕金森病相关的基因靶点,筛选了65条通路。结论:针刺治疗PD与刺激多巴胺分泌,减少多巴胺能神经元凋亡、调节5-羟色胺代谢水平、提高去甲肾上腺素水平、刺激神经营养因子、缓解炎症反应等密切相关。

Objective: To investigate the multi-target mechanism of acupuncture for Parkinson’s disease (PD) using text mining, bioinformatics, and network topology. Methods: Literature was searched to dis-cover active compounds produced after acupuncture, and potential targets of action were predicted by STITCH and SwissTargetPrediction databases. PD-associated disease gene targets were screened using the Human Gene Database, the Online Human Mendelian Inheritance Database, and the Glob-al Protein Resources Database. Cytoscape3.10.1 software was used to construct protein-protein in-teraction network maps of shared targets, and gene ontology enrichment analysis and Kyoto Ge-nome Encyclopedia enrichment analysis of shared targets were performed and visualized by DAVID database, respectively. Results: A total of 114 gene targets related to acupuncture for Parkinson’s disease were identified, and 65 pathways were screened. Conclusion: Acupuncture therapy for PD is closely related to stimulating dopamine secretion, reducing apoptosis of dopaminergic neurons, regulating 5-hydroxytryptamine metabolism levels, increasing norepinephrine levels, stimulating neurotrophic factors, and alleviating inflammatory responses.