抗EGFR单链抗体融合Gelonin免疫毒素与紫杉醇协同抗肿瘤作用及机制研究

Synergistic Cytotoxic Effect of Anti-EGFR/Gelonin Immunotoxin Combined with Taxol Anti-Tumor Effects and Its Mechanism

目的:研究抗EGFR单链抗体融合gelonin免疫毒素(E/rG)与紫杉醇是否具有协同抗肿瘤作用,进一步探讨两种药物联合作用产生协同效应的机制。方法:在体外实验中,利用MTT方法研究两种药物联合抑制EGFR阳性癌细胞的生物学效应;在体内试验中,建立人肺癌A549细胞裸鼠移植瘤模型,两种药物联合治疗评价两者的协同抗肿瘤作用。利用ELISA检测紫杉醇单独给药治疗后小鼠血清和肿瘤ECF中的EGFR水平。结果:在体外未检测到E/rG免疫毒素与紫杉醇的协同作用,在体内检测到两种药物具有显著的协同作用。ELISA检测结果显示紫杉醇治疗能够降低小鼠血清和肿瘤ECF中的EGFR水平。结论:在体外实验中,未发现两种药物协同抑制肿瘤细胞生长的作用,而在体内实验中,两种药物具有显著的协同抗肺癌肿瘤作用,其作用机制是紫杉醇给药后降低了小鼠血液中和肿瘤组织间隙液中的游离EGFR含量,减少了游离EGFR对免疫毒素的靶向抗肿瘤作用的影响。为抗EGFR单链抗体融合gelonin免疫毒素临床使用方案具有重要的指导意义。

Iron is an essential element for cell growth. The iron contents in tumor cells are higher than those in normal cells. The addition of exogenous iron can prevent apoptosis while iron chelators can inhibit the growth of tumor cells. Therefore, the anti-tumor effect of iron chelators obtains more and more extensive concern. This article aims to present a review on recent advancements in studies of iron and cervical cancer, and to explore the mechanism of iron in initiating cervical cancer as well as the effects of iron chelators on treatment of cervical cancer.