原位移植法建立肝脏H22肝癌细胞移植瘤小鼠模型

Orthotropic Transplantation to Establish H22 Hepatocellular Carcinoma Model in Mice

目的:建立H22肝癌细胞小鼠肝脏原位移植瘤模型,为进一步开展相关实验研究做准备。方法:直接注射一定数量的H22肝癌细胞于C57BL/6小鼠肝脏左叶,构建肝癌原位移植瘤模型。结果:术后7天小鼠肝脏表面出现大小不一结节散在分布,随着时间延长结节增多增大,后期腹腔脏器出现浸润、粘连,腹水增多。肝脏瘤组织及癌旁组织进行组织病理学检查,肝脏原位成瘤率100%,无自发消退。结论:原位注射H22肝癌细胞株能在肝脏局部形成原位移植瘤,且成瘤时间相对较短,成瘤过程中伴有脏器转移、腹水形成等,较好的模拟了机体原发性肝癌的病理生理学过程,能满足对肝癌相关研究的要求。

Objective: To establish a liver orthotropic transplantation tumor model with H22 cells, for the further development of the related experimental study. Methods: A certain number of H22 cells were directly injected in the left lobe of the liver in C57BL/6 mice, establishing a transplanted tu-mor model of hepatocellular carcinoma in situ. Results: After 7 days of surgery, the liver surface appeared irregular size nodules, nodules increased with the prolongation of time increases, the late abdominal organs appeared invasion, adhesion, ascites was increased. Histopathological ex-amination of liver tumor tissues and adjacent tissues of cancer showed that liver orthotropic transplantation tumor formation rate was 100%, no spontaneous regression. Conclusions: Ortho-tropic injection of H22 cell established orthotropic transplantation tumor formation in the liver, and the tumor formation time was relatively short, tumor formation processes associated with organ metastasis and ascites. This model was reasonable to simulate the pathological and physiological body of liver cancer in human, and could satisfy the requirement of research on liver cancer.