A549、MRC-5的有氧糖酵解及养阴扶正汤对A549有氧糖酵解的干预

Aerobic Glycolysis of A549 and MRC-5 and the Intervention of Yangyinfuzheng Decoction on Aerobic Glycolysis of A549

目的:探讨人肺腺癌细胞A549、人胚肺成纤维细胞MRC-5的糖酵解方式以及养阴扶正糖对人肺腺癌A549有氧糖酵解的干预。方法:应用3D的培养方式,在常氧、低氧、低氧缺糖的条件下,CCK8法检测细胞的增殖活性,细胞培养上清液的PH值、ELISA法检测葡萄糖的消耗、乳酸的生成。结果:在常氧、低氧、低氧缺糖的条件下,A549细胞OD值逐渐升高,MRC-5细胞OD值逐渐下降;A549细胞培养液的PH值略低于MRC-5细胞培养液的PH值;A549对葡萄糖的利用高于MRC-5细胞,A549细胞的乳酸生成量高于MRC-5细胞;养阴扶正能够抑制A549细胞的增殖,减少葡萄糖的消耗及乳酸、ATP的生成。结论:人肺腺癌A549细胞利用葡萄糖进行有氧糖酵解,养阴扶正汤可抑制人肺腺癌细胞A549的有氧糖酵解,进而抑制了人肺腺癌细胞A549的增殖。

Objective: To investigate the glycolysis of human lung adenocarcinoma cell A549 and human em-bryonic lung fibroblasts MRC-5 and the effect of Yangyinfuzheng sugar on the aerobic glycolysis of human lung adenocarcinoma cell A549. Methods: Under the condition of normoxia, hypoxia and hypoglycemia, the proliferation activity of cells was detected by CCK8 method, the PH value of cell culture supernatant, and the glucose consumption and lactic acid production were detected by ELISA. Results: Under normoxia, hypoxia and hypoglycemia, the OD value of A549 cells increased gradually, while that of MRC-5 cells decreased gradually;the PH value of A549 cell culture medium was slightly lower than that of MRC-5 cell culture medium;the glucose utilization of A549 cells was higher than that of MRC-5 cells, and the lactic acid production of A549 cells was higher than that of MRC-5 cells;the effect of nourishing Yin and nourishing Zheng could inhibit it. The proliferation of A549 cells was reduced, and glucose consumption and lactic acid and ATP production were reduced. Conclusion: The aerobic glycolysis of human lung adenocarcinoma A549 cells is carried out by glucose. Yangyinfuzheng decoction can inhibit the aerobic glycolysis of human lung adenocarcinoma A549 cells, and then inhibit the proliferation of human lung adenocarcinoma A549 cells.