SREBF2-AS1调控胆固醇合成代谢促进乳腺癌生长

SREBF2-AS1 Promotes the Growth of Breast Cancer via Regulating Cholesterol Anabolism

目的:研究SREBF2-AS1调控胆固醇合成代谢和SREBF2促进乳腺癌生长作用和分子机制。方法:qRT-PCR检测相关基因的转录水平。干扰SREBF2-AS1和过表达SREBF2后,MTT实验、BrdU实验和凋亡实验研究SREBF2-AS1在乳腺癌中作用及分子机制。结果:SREBF2-AS1在乳腺癌组织和乳腺癌细胞株中高表达。干扰掉SREBF2-AS1后抑制乳腺癌的生长和诱导其凋亡。进一步研究发现其通过调控SREBF2和胆固醇代谢进而调控乳腺癌生长。结论:SREBF2-AS1通过调控SREBF2和胆固醇代谢促进乳腺癌生长。

Objective: To study the molecular mechanism of SREBF2-AS1 regulating cholesterol anabolism and SREBF2 promoting the growth of breast cancer. Methods: The transcription level of Genes was detected by qRT PCR. After interference with SREBF2-AS1 and overexpression of SREBF2, MTT assay, BrdU assay and apoptosis assay were used to study the role and molecular mechanism of SREBF2- AS1 in breast cancer. Results: SREBF2-AS1 was highly expressed in breast cancer tissues and breast cell lines. Interference with SREBF2-AS1 inhibits the growth and induces apoptosis of breast cancer. Further studies found that it regulates the growth of breast cancer by regulating SREBF2 and cholesterol metabolism. Conclusion: SREBF2-AS1 can promote the growth of breast cancer by regulating SREBF2 and cholesterol metabolism.