紫斑牡丹内生真菌分离培养分子鉴定及抑菌活性研究

Study on Identification and Antimicrobial Activity of Endophytic Fungi Separation from Paeonia rockii

本研究对紫斑牡丹的根、茎、叶内生真菌进行分离培养,根据形态学特征、培养特性及rDNA-ITS序列分析相结合的方法对分离到的菌种进行鉴定,并对其与黄瓜枯萎病原菌尖孢镰刀菌Fusarium oxysporum同时接种进行抑菌试验,以筛选抑制尖孢镰刀菌Fusarium oxysporum的内生菌菌株。结果表明,从紫斑牡丹中获得20株内生真菌,经形态和分子共鉴定出11种,J2为菊拟茎点霉菌Paraphoma chrysanthemicola(Hollós) Gruyter,J6HUI为Aspergillus calidoustus Varga,J7HEI为马铃薯刺盘孢Colletotrichumcoccodes (Wallr.) S. Hughes,J8为木贼镰刀菌Fusarium equiseti (Corda) Sacc;G9无性型为多喙茎点霉Phoma multirostrata (P.N. Mathur, S.K. Menon & Thirum.) Dorenb. & Boerema,有性型为Boeremia sp.;G1、G6为番茄棘壳孢Pyrenochaeta lycopersici R.W. Schneid. & Gerlach,J1为Nodulosphaeria cirsii (P. Karst.) L. Holm,G5为Fusarium sp.,J6HUI2为Didymella glomerata (Corda)Qian Chen & L. Cai;J7HUI为Peyronellaea sp. (派伦霉属),J1和J6HUI为中国新记录种,其中4株具有抑菌作用,G5、G9、J1和J2对黄瓜枯萎病具有不同程度的抑制作用,平均抑菌率分别为57%、57.2%、28.8%和54.8%。