研究生实验教学中细胞转染技术结合斑点免疫印迹技术的实验探索

The Experimental Design of Cell Transfection Combined with Dot Immunoblotting for Experiment Teaching of Graduate Students

在研究生组织及细胞培养实验教学中,细胞转染技术是一个重要的组成部分,以往实验方案使用携带荧光蛋白GFP基因的质粒转染细胞后,可使用荧光显微镜进行观察定性分析转染是否成功并粗略计算转染效率,但对于没有荧光标记的细胞转染结果分析检测方法在教学上尚有欠缺。本实验针对这个问题,在检测方法中加入斑点免疫印迹技术,并对实验方案及分析方法进行了探索,以便于学生掌握更全面的细胞生物学分析技术。

In the experimental teaching of tissue and cell culture for graduate students, cell transfection tech-nology is an important component. In previous experimental schemes, plasmids carrying fluores-cent protein GFP genes were used to transfect cells. Fluorescence microscopy can be used to observe the results of transfection and roughly calculate transfection efficiency. However, there is still a lack of methods for analyzing and detecting transfection results of cells without fluorescent markers. This experiment aims to address this issue by incorporating dot immunoblotting technology into the detection method, and exploring the experimental protocol and analysis methods to help stu-dents to handle more comprehensive cell biology techniques.