TMAH浓度对生物硝化系统中微生物群落多样性的影响

Microbial Community Diversity Analysis of Nitrification Performance in Bioreactors with under TMAH Concentrations

本研究以高科技产业常用化学品氢氧化四甲基铵(Tetramethylammonium hydroxide, TMAH)作为研究目标,以生物处理方法进行TMAH生物急毒性减量,期能解决高科技产业废水生物急毒性议题。针对含TMAH的工艺废水,以好氧(Aerobic)反应槽进行探讨,设计TMAH浓度分别为0、1000、3000 mg∙L−1三个反应槽;实验结果,TMAH 0 mg∙L−1组呈现良好的硝化反应,微生物结构组成中浮霉菌门 (Planctomycetes) Gemmata属的比例比其他两组高。TMAH 1000 mg∙L−1组与TMAH 0 mg∙L−1组相比,TMAH 1000 mg∙L−1组增加了TMAH浓度的变异性,因而带入新的真菌物种(如:变形菌门(Proteobacteria) Brachymonas属、拟杆菌门(Bacteroidetes) Flavobacterium属),所以菌种丰富度也明显提高。TMAH 3000 mg∙L−1组菌种分布受到诸多因素(TMAH浓度高、硝化作用抑制)的综合制约影响,物种总量和丰度具有特异性,其中以变形菌门(Proteobacteria)中的Reyranella属数量明显比TMAH 0 mg∙L−1组及TMAH 1000 mg∙L−1组高。

This study used an aerobic batch reactor feeding TMAH (Tetramethylammonium hydroxide) con-taining synthetic wastewater to evaluate TMAH degradation and microbial community changes in the aerobic batch reactor through different influent TMAH concentration. Based on different in-fluent TMAH concentration (0, 1000, 3000 mg∙L−1), TMAH 0 mg∙L−1 showed a good nitrification re-action, and the proportion of Gemmata genus in the Planctomycetes was higher than that of the other two groups. With TMAH concentration increasing, the relative abundance of TMAH 1000 mg∙L−1 was high, and Proteobacteria and Bacteroidetes were the new species. Afterward, the dis-tribution of TMAH 3000 mg∙L−1 strains was affected by a combination of factors (high TMAH con-centration, nitrification inhibition), and the total amount and abundance of the species were specific. Among them, the Reyranella genus in Proteobacteria was obviously higher than TMAH 0 mg∙L−1 and TMAH 1000 mg∙L−1.