基于高效液相色谱–串联质谱法同时测定沉积物中磺胺类抗生素及其代谢物

Simultaneous Determination of Sulfonamide Antibiotics and Their Metabolites in Sediments by High Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了一种同时快速提取并检测沉积物中多种磺胺类抗生素及其典型代谢产物的方法,沉积物样品经冷冻干燥后,经萃取液(纯水,乙腈)萃取、净化盐(硫酸镁,C18)净化后,利用高效液相色谱–串联质谱仪(LC-MS/MS)进行测定。和常用的加速溶剂萃取法相比,该方法使用的有机溶剂量降低了57%,提取时间减少了40%。此外,该方法在低浓度下的回收率都相对较高,范围在为80%~121%,相对标准偏差小于9%。该方法可应用于水体沉积物中磺胺类抗生素及其代谢物的同时检测,且检测过程相对快速。

A rapid method was established to simultaneously extract and detect various sulfonamide antibiotics and their typical metabolites in sediments. After freeze-drying, the sediment samples were extracted with extraction solution (pure water, acetonitrile) and purified with purification salt (magnesium sulfate, C18). The target compounds were then measured by high performance liquid chromatography-tandem mass spectrometer (LC-MS/MS). Compared with the accelerated solvent extraction method, the used organic solvent in the present method was reduced by 57%, and the extraction time was reduced by 40%. In addition, the recoveries of the method at low concentrations were relatively high, ranging from 80% to 121%, and the relative standard deviations were all less than 9%. It suggests that the method could be applied to simultaneously detect sulfonamide antibiotics and their metabolites in sediments, and the detection process is relatively fast.