一株楚雄市区地表水体小球藻的产氧性能研究

Study on Oxygen Production Performance of Chlorella in Surface Water Body of Chuxiong Urban Area

为研究楚雄市区水体中小球藻的光合产氧性能,本文以一株研究区内分离培养的小球藻为研究对象,通过对培养基内N、P、K、微量元素进行浓度梯度设计,结合单因子实验和正交试验优化该小球藻产氧条件。研究结果显示该小球藻生长的最适N、P、K、微量元素浓度分别为53.62 mg/L、487.43 mg/L、110.59 mg/L、1.0 ml,小球藻的最佳产氧量为13.51 mg/L。该株小球藻在最适培养条件下产溶解氧达到过饱和,在实际应用及功能开发中具有巨大开发潜力。

In order to study the photosynthetic oxygen production performance of chlorella in the water body of Chuxiong urban area, this paper took chlorella isolated and cultured in the study area as the research object, and optimized the oxygen production conditions of chlorella by designing the concentration gradient of N, P, K and trace elements in the culture medium, combined with single factor experiments and orthogonal experiments. The results showed that the optimal concentra-tions of N, P, K and trace elements for chlorella growth were 53.62 mg/L, 487.43 mg/L, 110.59 mg/L and 1.0ml, respectively, and the optimal oxygen production of chlorella was 13.51mg/L. This strain of chlorella produces dissolved oxygen and reaches supersaturation under optimal culture conditions, which has great development potential in practical application and functional devel-opment.