摘要
<i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span><span style="font-family:;" "=""><span style="font-family:Verdana;"> used as an analgesic and anti-inflammatory. It is often used not only in Mongolian medicine but also in traditional Chinese medicine. However, there are few studies on its impact of inflammatory cytokines. This study explored the effects of </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i> <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> used human colon cancer (Caco-2) cells. Cytotoxicity was assessed by measuring effects on expression of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">, IL-6, IL-8, and TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> used enzyme-linked immun</span><span style="font-family:Verdana;">o</span><span style="font-family:;" "=""><span style="font-family:Verdana;">sorbent assay (ELISA). </span><span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">In vitro</span></i><span style="font-family:Verdana;"></span></i></span></span><span style="font-family:Verdana;"> study demonstrated that incubation of Caco-2 cells with some kinds of</span><i> </i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> at some concentrations inhibit secretion of TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> and FAXIU </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">ACONITUM</span></i> <i><span style="font-family:Verdana;">KUSNEZOFFII</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> could inhibit the secretion of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">. While IL-6 and IL-8 levels were unaffected after 24 h of treatment with </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">qujian </span><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">faxiu </span><i><i><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;">.</span></i></i></i></i>
<i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span><span style="font-family:;" "=""><span style="font-family:Verdana;"> used as an analgesic and anti-inflammatory. It is often used not only in Mongolian medicine but also in traditional Chinese medicine. However, there are few studies on its impact of inflammatory cytokines. This study explored the effects of </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i> <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> used human colon cancer (Caco-2) cells. Cytotoxicity was assessed by measuring effects on expression of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">, IL-6, IL-8, and TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> used enzyme-linked immun</span><span style="font-family:Verdana;">o</span><span style="font-family:;" "=""><span style="font-family:Verdana;">sorbent assay (ELISA). </span><span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">In vitro</span></i><span style="font-family:Verdana;"></span></i></span></span><span style="font-family:Verdana;"> study demonstrated that incubation of Caco-2 cells with some kinds of</span><i> </i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> at some concentrations inhibit secretion of TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> and FAXIU </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">ACONITUM</span></i> <i><span style="font-family:Verdana;">KUSNEZOFFII</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> could inhibit the secretion of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">. While IL-6 and IL-8 levels were unaffected after 24 h of treatment with </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">qujian </span><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">faxiu </span><i><i><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;">.</span></i></i></i></i>
