期刊文献+

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i>Infection

Efficacy of Some Antibiotics in Curing Resistant <i>Escherichia coli</i>Infection
下载PDF
导出
摘要 There is growing interest in re-evaluation of older antibiotics with the wide spread of pathogen resistance, especially gram negative bacteria, which impair treatment of some infections. In contrast various studies have reported that some antibiotics have efficacy in clearing resistant bacterial infections. On account of that it was interesting to evaluate the efficacy of erythromycin, chloramphenicol and/or tenoxicam in curing and/or relieving wound infection of highly resistant Escherichia coli and investigate the possible mechanisms beyond their antibacterial activity. This was achieved through evaluating highly resistant E. coli strains in vitro using agar dilution and in vivo rat models of E. coli infected wound and acute inflammation by carrageenin, where possible mechanisms were evaluated through measuring immunological mediators and histopathological examination. This study revealed that in vivo, erythromycin alone or in combination with tenoxicam significantly improved the healing of infected skin wounds with E. coli irresspective of resistancy in vitro. In addition to the improvement of immunological mediators involved in inflammatory reaction, oxidative stress and in cytokines expression as response to the bacterial infection in vivo. On the other hand chloramphenicol neither alone nor in combination with tenoxicam, achieved any significant effect. Tenoxicam didn’t show antimicrobial activity alone nor in combination with tested antibiotics in vitro, but it has shown synergestic activity in combination with tested antibiotics in vivo. Thus we concluded that immunomodulatory activity of erythromycin through anti-inflammatory and antioxidant effects was the possible mechanisms by which this antibiotic had healed infection with resistant E. coli in vivo, despite its resistancy to this antibiotic in vitro. There is growing interest in re-evaluation of older antibiotics with the wide spread of pathogen resistance, especially gram negative bacteria, which impair treatment of some infections. In contrast various studies have reported that some antibiotics have efficacy in clearing resistant bacterial infections. On account of that it was interesting to evaluate the efficacy of erythromycin, chloramphenicol and/or tenoxicam in curing and/or relieving wound infection of highly resistant Escherichia coli and investigate the possible mechanisms beyond their antibacterial activity. This was achieved through evaluating highly resistant E. coli strains in vitro using agar dilution and in vivo rat models of E. coli infected wound and acute inflammation by carrageenin, where possible mechanisms were evaluated through measuring immunological mediators and histopathological examination. This study revealed that in vivo, erythromycin alone or in combination with tenoxicam significantly improved the healing of infected skin wounds with E. coli irresspective of resistancy in vitro. In addition to the improvement of immunological mediators involved in inflammatory reaction, oxidative stress and in cytokines expression as response to the bacterial infection in vivo. On the other hand chloramphenicol neither alone nor in combination with tenoxicam, achieved any significant effect. Tenoxicam didn’t show antimicrobial activity alone nor in combination with tested antibiotics in vitro, but it has shown synergestic activity in combination with tested antibiotics in vivo. Thus we concluded that immunomodulatory activity of erythromycin through anti-inflammatory and antioxidant effects was the possible mechanisms by which this antibiotic had healed infection with resistant E. coli in vivo, despite its resistancy to this antibiotic in vitro.
出处 《Journal of Biosciences and Medicines》 2015年第7期31-53,共23页 生物科学与医学(英文)
关键词 EFFICACY ANTIBIOTICS RESISTANT E. coli INFECTION Efficacy Antibiotics Resistant E. coli Infection
  • 相关文献

参考文献1

二级参考文献8

  • 1何志义,邹朝霞,刘启才,李冰,钟南山,冉丕鑫.红霉素对支气管上皮细胞转录因子核因子κB、激活蛋白1活性的影响[J].中华结核和呼吸杂志,2004,27(9):637-638. 被引量:6
  • 2Volohonsky G,Tuby C,Porat N,et al. A spectrophotometric assay of-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells. Chemico Biolog Interactions, 2002,140:49-65.
  • 3Desaki M, Takizawa H, Ohtoshi T, et al. Erythromycin suppress nuclear factor-kappa B and activator protein-1 activation in human bronchial epithelial cells. Biochem Biophys Res Commun, 2000,267:124-128.
  • 4Abe S, Nakamura H, Inoue S, et al. Interleukin-8 gene repression by clarithromycin is mediated by the activator protein-1 binding site in human bronchial epithelial cells. Am J Respir Cell Mol Biol,2000,22:51-60.
  • 5Rahman I, Smith CA, Antonicelli F ,et al. Characterisation of γ-glutamylcysteine synthetase heavy subunit promoter:a critical role for AP-1. FEBS Lett,1998, 427:129-133.
  • 6Rahman I, MacNee W. Lung glutathione and oxidative stress:implications in cigarette smoke-induced airway disease. Am J Physiol, 1999,277 : L1067-1088.
  • 7Rahman I. Oxidative stress, chromatin remodeling and gene transcription in inflammation and chronic lung diseases. J Biochem Mol Biol,2003 ,36 :95-109.
  • 8Gotfried MH. Macrolides for the treatment of chronic sinusitis,asthma, and COPD. Chest, 2004,125:52S-61S.

共引文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部