摘要
We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). It was shown that platelet incubation with 20 - 75 <em>μ</em>M H<sub>2</sub>O<sub>2</sub> during 24 hours at 20<span style="white-space:nowrap;">°</span>C - 22<span style="white-space:nowrap;">°</span>C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 <em>μ</em>M H<sub>2</sub>O<sub>2</sub>. 30 - 60 μM H<sub>2</sub>O<sub>2</sub> dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H<sub>2</sub>O<sub>2</sub>. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.
We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). It was shown that platelet incubation with 20 - 75 <em>μ</em>M H<sub>2</sub>O<sub>2</sub> during 24 hours at 20<span style="white-space:nowrap;">°</span>C - 22<span style="white-space:nowrap;">°</span>C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 <em>μ</em>M H<sub>2</sub>O<sub>2</sub>. 30 - 60 μM H<sub>2</sub>O<sub>2</sub> dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H<sub>2</sub>O<sub>2</sub>. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.
作者
Maksim S. Makarov
Maya V. Storozheva
Maksim S. Makarov;Maya V. Storozheva(Laboratory of Biotechnologies and Transfusiology, N. V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow, Russia)
