摘要
The industrial importance and the high cost of the commercial amylase require the study of microorganisms that produce these enzymes. For this reason, the objective of this work was to isolate filamentous fungi from a region of the caatinga and evaluate their potential for the production of amylase. Four soil samples were collected from a deactivated dump located in the city of Diamantina, MG, in a region of the caatinga. The analysis of amylolytic production in a submerged medium at the ideal temperature of each microorganism was performed using the saccharification method, and the reducing sugars formed were quantified by DNS. Fourteen filamentous fungi were isolated, which had different morphological aspects. Regarding amylase production, a mean activity of 0.477 U<span style="white-space:nowrap;">·</span>mL<sup>-1</sup> was obtained with the isolates I 1.2.1 and I 4.4.1. These results bring important information regarding the biodiversity of the caatinga, in addition to the isolation of microorganisms that can be used as biological machinery to obtain metabolites with high biotechnological and industrial potential.
The industrial importance and the high cost of the commercial amylase require the study of microorganisms that produce these enzymes. For this reason, the objective of this work was to isolate filamentous fungi from a region of the caatinga and evaluate their potential for the production of amylase. Four soil samples were collected from a deactivated dump located in the city of Diamantina, MG, in a region of the caatinga. The analysis of amylolytic production in a submerged medium at the ideal temperature of each microorganism was performed using the saccharification method, and the reducing sugars formed were quantified by DNS. Fourteen filamentous fungi were isolated, which had different morphological aspects. Regarding amylase production, a mean activity of 0.477 U<span style="white-space:nowrap;">·</span>mL<sup>-1</sup> was obtained with the isolates I 1.2.1 and I 4.4.1. These results bring important information regarding the biodiversity of the caatinga, in addition to the isolation of microorganisms that can be used as biological machinery to obtain metabolites with high biotechnological and industrial potential.
作者
Ingrid Cristina Soares Amorim
Gessica Oliveira Marinho
Tarcisio Michael Ferreira Soares de Oliveira
Juan Pedro Bretas Roa
Arlete Barbosa dos Reis
David Lee Nelson
Thiago Machado Pasin
Vivian Machado Benassi
Ingrid Cristina Soares Amorim;Gessica Oliveira Marinho;Tarcisio Michael Ferreira Soares de Oliveira;Juan Pedro Bretas Roa;Arlete Barbosa dos Reis;David Lee Nelson;Thiago Machado Pasin;Vivian Machado Benassi(Instituto de Ciência e Tecnologia, Universidade Federal dos Vales dos Jequitinhonha e Mucuri, Campus JK, Diamantina, Brasil;Programa de Pós-Graduação em Biocombustíveis, Universidade Federal dos Vales dos Jequitinhonha e Mucuri, Campus JK, Diamantina, Brasil;Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brasil)