摘要
Oral antibiotics are often prescribed to prevent infection after implant surgery;however, only a small fraction of the antibiotics can reach the implants. Thus, there are concerns about overusing antibiotics. We designed and fabricated porous implants with interconnecting 3D structures (I3D) and hypothesized that such I3D structures could serve as a depository for antimicrobial agents to prevent infection locally. The implants were either treated with antibiotics or coated with silver nanoparticles (AgNPs) by electrodeposition to test this hypothesis. The antimicrobial assay was conducted, and bacterial growth zones of inhibition (ZOIs) were monitored. Overall, I3D implants resulted in larger ZOIs than did the solid implants, and the center I3D (cI3D)-implant produced the largest ZOI. In the antibiotic treatment testing, the diameters of ZOIs of the solid implant vs. I3D implant were about 14 mm vs. 15 to 18 mm on day 2;however, the diameter quickly reduced to 9 mm on day 3 and 5 mm on days 6 and 8 for the solid implant, while no obvious change of the zone was seen for I3D implants. For the AgNPs coated implants, the ZOIs for the I3D implants were generally greater than the solid implant over four weeks of incubation. A significantly larger ZOI (~1 - 2 mm larger on average) was seen for AgNPs coated I3D implants at 0.1 V - 0.01 M, 0.3 V - 0.01 M, and 1.5 V - 0.01 M treatments compared to AgNPs coated solid implants. Given that we have previously shown that I3D implants can reserve chemoattractants to recruit stem cells to enhance osseointegration, we conclude that implants with the I3D structures could be beneficial not only for osseointegration but also in preventing infection.
Oral antibiotics are often prescribed to prevent infection after implant surgery;however, only a small fraction of the antibiotics can reach the implants. Thus, there are concerns about overusing antibiotics. We designed and fabricated porous implants with interconnecting 3D structures (I3D) and hypothesized that such I3D structures could serve as a depository for antimicrobial agents to prevent infection locally. The implants were either treated with antibiotics or coated with silver nanoparticles (AgNPs) by electrodeposition to test this hypothesis. The antimicrobial assay was conducted, and bacterial growth zones of inhibition (ZOIs) were monitored. Overall, I3D implants resulted in larger ZOIs than did the solid implants, and the center I3D (cI3D)-implant produced the largest ZOI. In the antibiotic treatment testing, the diameters of ZOIs of the solid implant vs. I3D implant were about 14 mm vs. 15 to 18 mm on day 2;however, the diameter quickly reduced to 9 mm on day 3 and 5 mm on days 6 and 8 for the solid implant, while no obvious change of the zone was seen for I3D implants. For the AgNPs coated implants, the ZOIs for the I3D implants were generally greater than the solid implant over four weeks of incubation. A significantly larger ZOI (~1 - 2 mm larger on average) was seen for AgNPs coated I3D implants at 0.1 V - 0.01 M, 0.3 V - 0.01 M, and 1.5 V - 0.01 M treatments compared to AgNPs coated solid implants. Given that we have previously shown that I3D implants can reserve chemoattractants to recruit stem cells to enhance osseointegration, we conclude that implants with the I3D structures could be beneficial not only for osseointegration but also in preventing infection.
作者
Steven Guo
Alexandria Ramos
Zhongqiang Li
Hong Yao
Jian Xu
Shaomian Yao
Steven Guo;Alexandria Ramos;Zhongqiang Li;Hong Yao;Jian Xu;Shaomian Yao(Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA;Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA;Division of Electrical and Computer Engineering, College of Engineering, Louisiana State University, Baton Rouge, LA, USA;Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA, USA)
