摘要
<strong>Objective: </strong>The aim of the study is to identify whether Atypical Lymphocyte (AL), liver transaminases, and Glutathione Reductase (GR) can be used as potential biomarkers in the assessment of severity and thrombocytopenia in dengue. <strong>Methods:</strong> A cross-sectional analytical study was carried out on diagnosed dengue patients admitted to Nawaloka Hospital, Sri Lanka. Blood samples were taken from patients (n = 50) on the day of admission, 3<sup>rd</sup> and 5<sup>th</sup> day from admission for analysis of GR, aspartate transaminase, alanine transaminase, platelets, white blood cells, and Atypical Lymphocytes (AL). <strong>Results:</strong> GR level of all three measured stages had a higher area under the curve (>88%), high sensitivity and specificity compared to liver transaminases. A significant regression model represents on admission GR and AL levels as predictive variables to platelet levels in day 03 from admission (Day 3 Platelet level = 127155.3 - 383 * GR - 0.431 * AL). <strong>Conclusion:</strong> Liver transaminases, GR, and AL% can be considered as a profile of predictive biomarkers in early diagnosis of severity of dengue infection. The degree of thrombocytopenia can be predicted using on admission GR and AL% level in acute dengue viral infection.
<strong>Objective: </strong>The aim of the study is to identify whether Atypical Lymphocyte (AL), liver transaminases, and Glutathione Reductase (GR) can be used as potential biomarkers in the assessment of severity and thrombocytopenia in dengue. <strong>Methods:</strong> A cross-sectional analytical study was carried out on diagnosed dengue patients admitted to Nawaloka Hospital, Sri Lanka. Blood samples were taken from patients (n = 50) on the day of admission, 3<sup>rd</sup> and 5<sup>th</sup> day from admission for analysis of GR, aspartate transaminase, alanine transaminase, platelets, white blood cells, and Atypical Lymphocytes (AL). <strong>Results:</strong> GR level of all three measured stages had a higher area under the curve (>88%), high sensitivity and specificity compared to liver transaminases. A significant regression model represents on admission GR and AL levels as predictive variables to platelet levels in day 03 from admission (Day 3 Platelet level = 127155.3 - 383 * GR - 0.431 * AL). <strong>Conclusion:</strong> Liver transaminases, GR, and AL% can be considered as a profile of predictive biomarkers in early diagnosis of severity of dengue infection. The degree of thrombocytopenia can be predicted using on admission GR and AL% level in acute dengue viral infection.
作者
Samantha Bandara
Lal G. Chandrasena
Hemantha Peiris
Visula Abesuriya
Arjuna De Silva
Chandima De Mel
Sanjay De Mel
Suranjith L. Seneviratne
Samantha Bandara;Lal G. Chandrasena;Hemantha Peiris;Visula Abesuriya;Arjuna De Silva;Chandima De Mel;Sanjay De Mel;Suranjith L. Seneviratne(Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka;Nawaloka Hospitals Research and Education Foundation, Nawaloka Hospitals PLC, Colombo, Sri Lanka;Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka;Department of Medicine, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka;Department of Haematology-Oncology, National University Cancer Institute, National University Health System, Singapore;Institute of Immunity and Transplantation, Royal Free Hospital and University College, London, UK)
