摘要
<span>Chlorhexidine is widely used in dentistry to treat various gingival conditions. Its side effects are widely described. Histologically, the gingiva consists of a stratified squamous epithelium, with a predominance of keratinocytes, the latter being fundamental in healing processes. Thus, the objective of this </span><i><span>in vitro</span></i><span><span> study was to evaluate the effects of a new product with active oxygen (blue<sup>®</sup></span><span>m) on keratinocytes. Keratinocytes (HACAT) were incubated with different concentrations of blue</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span></span><span>m (1, 10 and 100 μl/ml), and another well was used as a control, without the presence of mouthwash. After 24, 48 and 72 hours, cell proliferation was analyzed by CyQUANT</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><sup style="vertical-align:super;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></sup></span></span></span></span></span></span></span></span><span><span><span>. It was possible to observe that lower concentrations (1 μl/ml) of blue</span><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span><span></span></span><span>m increased cell proliferation in HACAT cell lines, while moderate and higher concentrations of mouthwash may present a cytotoxic effect. This is the first </span></span></span><i><span>in vitro</span></i><span> study with show</span><span>ing</span><span><span> that human keratinocytes cell line demonstrated greater proliferation rate when exposed to lower concentrations of blue</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span><span>m mouthwash.</span></span>
<span>Chlorhexidine is widely used in dentistry to treat various gingival conditions. Its side effects are widely described. Histologically, the gingiva consists of a stratified squamous epithelium, with a predominance of keratinocytes, the latter being fundamental in healing processes. Thus, the objective of this </span><i><span>in vitro</span></i><span><span> study was to evaluate the effects of a new product with active oxygen (blue<sup>®</sup></span><span>m) on keratinocytes. Keratinocytes (HACAT) were incubated with different concentrations of blue</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span></span><span>m (1, 10 and 100 μl/ml), and another well was used as a control, without the presence of mouthwash. After 24, 48 and 72 hours, cell proliferation was analyzed by CyQUANT</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><sup style="vertical-align:super;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></sup></span></span></span></span></span></span></span></span><span><span><span>. It was possible to observe that lower concentrations (1 μl/ml) of blue</span><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span><span></span></span><span>m increased cell proliferation in HACAT cell lines, while moderate and higher concentrations of mouthwash may present a cytotoxic effect. This is the first </span></span></span><i><span>in vitro</span></i><span> study with show</span><span>ing</span><span><span> that human keratinocytes cell line demonstrated greater proliferation rate when exposed to lower concentrations of blue</span><span style="color:#4F4F4F;"><span style="color:#4F4F4F;"><span style="color:#393939;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:normal;"><span style="white-space:nowrap;"><span style="white-space:nowrap;"><sup><span style="font-size:10px;white-space:normal;">®</span><span style="font-size:10px;white-space:normal;"></span></sup></span></span></span></span></span></span></span></span><span>m mouthwash.</span></span>
作者
Bruna Marca Mattei
Soraia A. W. Imanishi
Grasieli de Oliveira Ramos
Paloma Santos de Campos
Suyany Gabriely Weiss
Tatiana Miranda Deliberador
Bruna Marca Mattei;Soraia A. W. Imanishi;Grasieli de Oliveira Ramos;Paloma Santos de Campos;Suyany Gabriely Weiss;Tatiana Miranda Deliberador(Department of Dentistry, Universidade do Oeste de Santa Catarina (UNOESC), Joaçaba, Brazil;Department of Dentistry, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;School of Health Sciences, Department of Dentistry, Universidade Positivo, Curitiba, Brazil)