摘要
Tricholoma giganteum also being called Tricholoma lobayense, belongs to Basidiomycotina, Hymenomycetes, Agaricales, Tricholomataceae and Tricholoma. It has high economic and medicinal values and is a kind of rare edible mushroom. Modern pharmacological studies have shown that T. giganteum have antitumor, antioxidant, antimicrobial and free radicals scavenging activity, and it can inhibit high blood pressure and HIV-1 reverse transcriptase. In this study, the technologies of isolation and purification of polysaccharides of T. giganteum were systematically studied. T. giganteum polysaccharides were obtained by hot-water extraction method. The infection of the extraction temperature, the extraction time, the solvent-solid ratio and the volume of solvent used during precipitation on the polysaccharides extraction ratio were studied. The extraction process was optimized by the uniform design (UD) method on the basis of the results of the single-factor experiments. The optimum extraction conditions of the mushroom polysaccharides (MP) were as follows: Extraction temperature was 100℃;extraction time was 3 hours;solvent-solid ratio was 20:1 and 5 times volume of 95% ethanol. The yield of polysaccharides of T. giganteum was 12.92%.
Tricholoma giganteum also being called Tricholoma lobayense, belongs to Basidiomycotina, Hymenomycetes, Agaricales, Tricholomataceae and Tricholoma. It has high economic and medicinal values and is a kind of rare edible mushroom. Modern pharmacological studies have shown that T. giganteum have antitumor, antioxidant, antimicrobial and free radicals scavenging activity, and it can inhibit high blood pressure and HIV-1 reverse transcriptase. In this study, the technologies of isolation and purification of polysaccharides of T. giganteum were systematically studied. T. giganteum polysaccharides were obtained by hot-water extraction method. The infection of the extraction temperature, the extraction time, the solvent-solid ratio and the volume of solvent used during precipitation on the polysaccharides extraction ratio were studied. The extraction process was optimized by the uniform design (UD) method on the basis of the results of the single-factor experiments. The optimum extraction conditions of the mushroom polysaccharides (MP) were as follows: Extraction temperature was 100℃;extraction time was 3 hours;solvent-solid ratio was 20:1 and 5 times volume of 95% ethanol. The yield of polysaccharides of T. giganteum was 12.92%.
