摘要
<span style="font-family:Verdana;">The current study evaluated the antioxidant, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase inhibitory and anti-</span><i><span style="font-family:Verdana;">Staphylococcus</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities of the fruiting bodies of </span><i><span style="font-family:Verdana;">Ganoderma</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;">. Methanolic extract of </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> (MEGL) was investigated for its </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activity using 1,1-diphenyl 2-picrylhydrazyl (DPPH) assay and EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value was determined. </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-Amylase inhibitory and anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities were carried out by conventional methods. The mushroom extract showed maximum free radical scavenging activity at a dose of 500 μg/mL with EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 22.1399 μg/mL. EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value for the inhibition of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase activity was found 22.6011 μg/mL. </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> extracts exhibited a concentration-dependent Anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activity. The vast differences of this inhibition between lower and higher doses were assessed significantly (p < 0.05). This study shows that the fruiting body of </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> has moderate antioxidant, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase inhibitory, and anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities. Thus this mushroom may be the alternative plant source for the antioxidant, anti-diabetic and antibacterial agents and further </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vivo</span></i><span style="font-family:Verdana;"> studies and isolation of bioactive compounds are required.</span>
<span style="font-family:Verdana;">The current study evaluated the antioxidant, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase inhibitory and anti-</span><i><span style="font-family:Verdana;">Staphylococcus</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities of the fruiting bodies of </span><i><span style="font-family:Verdana;">Ganoderma</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;">. Methanolic extract of </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> (MEGL) was investigated for its </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activity using 1,1-diphenyl 2-picrylhydrazyl (DPPH) assay and EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value was determined. </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-Amylase inhibitory and anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities were carried out by conventional methods. The mushroom extract showed maximum free radical scavenging activity at a dose of 500 μg/mL with EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 22.1399 μg/mL. EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value for the inhibition of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase activity was found 22.6011 μg/mL. </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> extracts exhibited a concentration-dependent Anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activity. The vast differences of this inhibition between lower and higher doses were assessed significantly (p < 0.05). This study shows that the fruiting body of </span><i><span style="font-family:Verdana;">G.</span></i> <i><span style="font-family:Verdana;">lucidum</span></i><span style="font-family:Verdana;"> has moderate antioxidant, </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-amylase inhibitory, and anti-</span><i><span style="font-family:Verdana;">S.</span></i> <i><span style="font-family:Verdana;">aureus</span></i><span style="font-family:Verdana;"> activities. Thus this mushroom may be the alternative plant source for the antioxidant, anti-diabetic and antibacterial agents and further </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vivo</span></i><span style="font-family:Verdana;"> studies and isolation of bioactive compounds are required.</span>
作者
Md. Moyan Uddin PK
Mohammad Sayful Islam
Shariful Haque
Lutfa Akther
Rumana Pervin
Md. Moyan Uddin PK;Mohammad Sayful Islam;Shariful Haque;Lutfa Akther;Rumana Pervin(Institute of Biological Science, University of Rajshahi, Rajshahi, Bangladesh;Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh;School of Science, Independent University of Bangladesh, Dhaka, Bangladesh;Department of Pharmacy, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh;Department of Biochemistry, Asahikawa Medical University, Hokkaido, Japan;Department of Pharmacy, Pabna University of Science and Technology, Pabna, Bangladesh;RHSTEP, Dhaka Medical College Hospital, Dhaka, Bangladesh)
