摘要
We attempted to study the role of HLA HLA-A, B, DRB1 and DQB1 in HIV-1 patient’s co infected with pulmonary tuberculosis (PTB). A total of 102 HIV-1 + patients co-infected with pulmonary tuberculosis and 200 healthy controls were included in HLA analysis. HLA-A*, HLA-B* HLA-DRB1* and DQB1* typing was done molecularly by PCR- SSOP (Polymerase Chain reaction-Sequence Specific Oligonucleotide Probing) method using kit (Dynal Kit – Invitrogen). The frequencies of the HLA-A, B HLA-DRB,1 and DQB1 alleles were determined using standard software. The HLA alleles identified among HIV + ve/PTB + ve co-infected patients as compared with healthy controls showed a significantly increased frequency of HLA-B*08:01:01 in HIV + ve/PTB + ve co-infected patients when compared with healthy controls (p = 0.011, OR 3.335, 95% CI 1.35-8.18), Likewise HLA-DQB1*03:01:03 was significantly increased in HIV + ve/PTB + ve co-infected patients as against healthy controls (p < 0.0001, OR 107.5, 95% CI 6.195 - 1865.3). Similarly HLA-DQB*06:01:02 allele frequency was observed in HIV + ve/PTB + ve co-infected patients as against healthy controls (p = 0.003, OR 4.808, 95% CI 1.72-13.39), HLA-DQB1*03:01:01 (p = 0.045, OR 0.219, 95% CI 0.051 - 0.940), HLA-DQB1*06:01:01:01 (p = 0.012, OR 0.334, 95% CI 0.145 - 0.770), alleles in HIV + ve/PTB + ve co-infected patients when compared with healthy controls. We can be concluded that different HLA alleles may render susceptibility or protection to in different ethnic population.
We attempted to study the role of HLA HLA-A, B, DRB1 and DQB1 in HIV-1 patient’s co infected with pulmonary tuberculosis (PTB). A total of 102 HIV-1 + patients co-infected with pulmonary tuberculosis and 200 healthy controls were included in HLA analysis. HLA-A*, HLA-B* HLA-DRB1* and DQB1* typing was done molecularly by PCR- SSOP (Polymerase Chain reaction-Sequence Specific Oligonucleotide Probing) method using kit (Dynal Kit – Invitrogen). The frequencies of the HLA-A, B HLA-DRB,1 and DQB1 alleles were determined using standard software. The HLA alleles identified among HIV + ve/PTB + ve co-infected patients as compared with healthy controls showed a significantly increased frequency of HLA-B*08:01:01 in HIV + ve/PTB + ve co-infected patients when compared with healthy controls (p = 0.011, OR 3.335, 95% CI 1.35-8.18), Likewise HLA-DQB1*03:01:03 was significantly increased in HIV + ve/PTB + ve co-infected patients as against healthy controls (p < 0.0001, OR 107.5, 95% CI 6.195 - 1865.3). Similarly HLA-DQB*06:01:02 allele frequency was observed in HIV + ve/PTB + ve co-infected patients as against healthy controls (p = 0.003, OR 4.808, 95% CI 1.72-13.39), HLA-DQB1*03:01:01 (p = 0.045, OR 0.219, 95% CI 0.051 - 0.940), HLA-DQB1*06:01:01:01 (p = 0.012, OR 0.334, 95% CI 0.145 - 0.770), alleles in HIV + ve/PTB + ve co-infected patients when compared with healthy controls. We can be concluded that different HLA alleles may render susceptibility or protection to in different ethnic population.
