摘要
Bunchosia glanduliera stands out because of the high content of flavonoid compounds in the pulp, contributing to the antioxidant potential of fruit extracts. Another plant species rich in flavonoid compounds is Markhamia tomentosa. However, to perform such an assay, a high-cost instrument is needed. To develop a simple and low-cost method for the determination of flavonoid compounds in M. tomentosa and B. glandulifera with PhotoMetrix<sup>®</sup> program application use pixels of digital imaging as an alternative method and linear correlation techniques for univariate analysis implementing systems of RGB colors (red, green, and blue). To determine the total flavonoids, the reaction with ferric chloride and quercetin was used as a control. For the acquisition of data or smartphones, low-cost materials were used, demonstrating the applicability of this analytical tool while comparing its cost to other analytical instrumentation. The total flavonoid content was also determined using a spectrophotometry technique in the visible ultraviolet spectrum (UV-Vis). The pulp of B. glandulifera showed the highest content of flavonoid compounds. The content of flavonoid compounds found in the fruit of B. glandulifera was 259.54 mg 100 g<sup>-1</sup>. In relation to the results found in the analysis of total flavonoids of M. tomentosa can be observed in the flower in natura has a higher content of these compounds. The use of PhotoMetrix<sup>®</sup> for the determination of flavonoid compounds in M. tomentosa and B. glandulifera reduced expense and analysis time. The method is reproducible and efficient. The proposed method can be adopted in different species.
Bunchosia glanduliera stands out because of the high content of flavonoid compounds in the pulp, contributing to the antioxidant potential of fruit extracts. Another plant species rich in flavonoid compounds is Markhamia tomentosa. However, to perform such an assay, a high-cost instrument is needed. To develop a simple and low-cost method for the determination of flavonoid compounds in M. tomentosa and B. glandulifera with PhotoMetrix<sup>®</sup> program application use pixels of digital imaging as an alternative method and linear correlation techniques for univariate analysis implementing systems of RGB colors (red, green, and blue). To determine the total flavonoids, the reaction with ferric chloride and quercetin was used as a control. For the acquisition of data or smartphones, low-cost materials were used, demonstrating the applicability of this analytical tool while comparing its cost to other analytical instrumentation. The total flavonoid content was also determined using a spectrophotometry technique in the visible ultraviolet spectrum (UV-Vis). The pulp of B. glandulifera showed the highest content of flavonoid compounds. The content of flavonoid compounds found in the fruit of B. glandulifera was 259.54 mg 100 g<sup>-1</sup>. In relation to the results found in the analysis of total flavonoids of M. tomentosa can be observed in the flower in natura has a higher content of these compounds. The use of PhotoMetrix<sup>®</sup> for the determination of flavonoid compounds in M. tomentosa and B. glandulifera reduced expense and analysis time. The method is reproducible and efficient. The proposed method can be adopted in different species.
