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Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging

Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging
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摘要 Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is one of the challenges researchers have been facing. In this context, this paper illustrates a simple dual modality high resolution flexible probe imaging system for imaging applications in micro/optofluidic channels. The proposed system exhibits axial and lateral resolution of about 16 μm and 3.12 μm respectively. This proposed system also exhibits a modulation transfer function (MTF) of about 38.42 %. The performance of the system is validated by imaging micro particles in a microchannel and obtaining the fluorescent emission spectrum simultaneously. Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is one of the challenges researchers have been facing. In this context, this paper illustrates a simple dual modality high resolution flexible probe imaging system for imaging applications in micro/optofluidic channels. The proposed system exhibits axial and lateral resolution of about 16 μm and 3.12 μm respectively. This proposed system also exhibits a modulation transfer function (MTF) of about 38.42 %. The performance of the system is validated by imaging micro particles in a microchannel and obtaining the fluorescent emission spectrum simultaneously.
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出处 《Optics and Photonics Journal》 2011年第4期197-203,共7页 光学与光子学期刊(英文)
关键词 MICROSCOPY Image Fiber SPECTROPHOTOMETER MICROCHANNEL Microscopy Image Fiber Spectrophotometer Microchannel
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