Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells 被引量：1

Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells

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摘要 Three transfection reagents, Lipofectamine&reg 2000, TransIT-PRO&reg and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO&reg was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI. Three transfection reagents, Lipofectamine&reg 2000, TransIT-PRO&reg and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO&reg was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.

作者 Si Nga Sou Karen M. Polizzi Cleo Kontoravdi

机构地区 Centre for Process Systems Engineering Department of Life Sciences Centre for Synthetic Biology and Innovation Department of Life Sciences Centre for Synthetic Biology and Innovation Centre for Process Systems Engineering

出处《Advances in Bioscience and Biotechnology》 2013年第12期1013-1019,共7页 生命科学与技术进展（英文）

关键词 TRANSIENT GENE Expression TRANSFECTION Efficiency Chinese HAMSTER OVARY Cells mRNA Stability Temperature SHIFT Transient Gene Expression Transfection Efficiency Chinese Hamster Ovary Cells mRNA Stability Temperature Shift

分类号 R73 [医药卫生—肿瘤]

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2013年第12期

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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells 被引量：1

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