摘要
Solid-state fermentation was carried out using mycelium powder of <em>Aspergillus niger</em> as substrate for the production of chitosanase of <em>Streptomyces</em>. Results of the experiments indicated that the optimal medium consisted of wheat bran and mycelium powder of <em>Aspergillus niger</em> with initial moisture content of 60% - 70%. The enzyme activity reached 41.33 U per gram dry medium after cultured for 5 days at 28<span style="white-space:nowrap;">°</span>C - 30<span style="white-space:nowrap;">°</span>C and an initial pH 6.5. Chitosanase was detected on the second day of incubation and had maximal activity at 5 days and decreased gradually within a 1 month period. Solid-state fermentation is maybe an economic alternative in the production.
Solid-state fermentation was carried out using mycelium powder of <em>Aspergillus niger</em> as substrate for the production of chitosanase of <em>Streptomyces</em>. Results of the experiments indicated that the optimal medium consisted of wheat bran and mycelium powder of <em>Aspergillus niger</em> with initial moisture content of 60% - 70%. The enzyme activity reached 41.33 U per gram dry medium after cultured for 5 days at 28<span style="white-space:nowrap;">°</span>C - 30<span style="white-space:nowrap;">°</span>C and an initial pH 6.5. Chitosanase was detected on the second day of incubation and had maximal activity at 5 days and decreased gradually within a 1 month period. Solid-state fermentation is maybe an economic alternative in the production.
