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Cocoa Powder as Delivery Medium for Probiotic Lactobacillus Strains

Cocoa Powder as Delivery Medium for Probiotic Lactobacillus Strains
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摘要 Three Lactobacillus strains previously isolated from artisanal Italian cheeses and identified by species-specific PCR as L. helveticus, L. paracasei and L. rhamnosus, were evaluated for the presence of functional traits, such as acidifying activity, cell surface hydrophobicity, antibiotic resistance, survival in low pH and in presence of bile salts, in comparison with two commercially available probiotic strains (Lactobacillus acidophilus La-5 and L. rhamnosus GG). Subsequently, with the aim to develop a new non-dairy functional product, cocoa powder was used as a medium for incorporating freeze-dried cultures of each tested strain and survival at different time/temperature conditions was investigated. The results obtained demonstrated that artisanal dairy products are interesting sources of new probiotic strains;in particular, the dairy origin strain L. rhamnosus showed a good probiotic performance and the highest level of survival during storage. Finally, we showed that cocoa powder represents a good delivery medium for lactobacilli: it could be considered a novel functional food exhibiting high antioxidant power and presenting probiotic potential. Three Lactobacillus strains previously isolated from artisanal Italian cheeses and identified by species-specific PCR as L. helveticus, L. paracasei and L. rhamnosus, were evaluated for the presence of functional traits, such as acidifying activity, cell surface hydrophobicity, antibiotic resistance, survival in low pH and in presence of bile salts, in comparison with two commercially available probiotic strains (Lactobacillus acidophilus La-5 and L. rhamnosus GG). Subsequently, with the aim to develop a new non-dairy functional product, cocoa powder was used as a medium for incorporating freeze-dried cultures of each tested strain and survival at different time/temperature conditions was investigated. The results obtained demonstrated that artisanal dairy products are interesting sources of new probiotic strains;in particular, the dairy origin strain L. rhamnosus showed a good probiotic performance and the highest level of survival during storage. Finally, we showed that cocoa powder represents a good delivery medium for lactobacilli: it could be considered a novel functional food exhibiting high antioxidant power and presenting probiotic potential.
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出处 《Advances in Microbiology》 2011年第1期1-6,共6页 微生物学(英文)
关键词 LACTOBACILLI PROBIOTICS Cocoa POWDER Lactobacilli Probiotics Cocoa Powder
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