摘要
Five castrated male Iberian pigs (100 ± 2 kg b.w.) fitted with T-shaped ileal cannulas at the terminal ileum were used to determine the effects of legume feeding on intestinal microbiota composition. The diets were based on defatted soybean (Glycine max), lupin (Lupinus angustifolius) or chickpea (Cicer arietinum) seed meals and contained similar amounts of digestible energy (14.2 - 15.1 MJ·kg-1) and protein (107 g·kg-1). A hydrolyzed casein diet was used to determine the bacterial counts in pigs fed on a vegetable-free diet. The composition of the intestinal microbiota at the terminal ileum was analysed by q-PCR. Higher (P 10 number of copies was determined in the ileal contents of pigs fed on lupin- or chickpea-based diets with respect to those fed on the soybean-based diet. Bacteroides and the Clostridium coccoides/Eubacterium rectale group log10 number of copies was lower (P < 0.01) than that of soybean in the ileal contents of chickpea-fed pigs. Enterobacteria and the Escherichia/Shigella group log10 number of copies was lower (P < 0.01) than that of soybean in pigs fed on diets based on lupin or chickpea. The number of copies of the different bacterial groups in animals fed on the casein-based diet was lower (P < 0.01) than that of soybean for lactobacilli and bacteroides, but was higher than that of soybean for bifidobacteria, enterobacteria and the Escherichia/Shigella group. This information suggests that lupin or chickpea feeding might induce a benefit in the microbiota composition of Iberian pigs in their final productive stages.
Five castrated male Iberian pigs (100 ± 2 kg b.w.) fitted with T-shaped ileal cannulas at the terminal ileum were used to determine the effects of legume feeding on intestinal microbiota composition. The diets were based on defatted soybean (Glycine max), lupin (Lupinus angustifolius) or chickpea (Cicer arietinum) seed meals and contained similar amounts of digestible energy (14.2 - 15.1 MJ·kg-1) and protein (107 g·kg-1). A hydrolyzed casein diet was used to determine the bacterial counts in pigs fed on a vegetable-free diet. The composition of the intestinal microbiota at the terminal ileum was analysed by q-PCR. Higher (P 10 number of copies was determined in the ileal contents of pigs fed on lupin- or chickpea-based diets with respect to those fed on the soybean-based diet. Bacteroides and the Clostridium coccoides/Eubacterium rectale group log10 number of copies was lower (P < 0.01) than that of soybean in the ileal contents of chickpea-fed pigs. Enterobacteria and the Escherichia/Shigella group log10 number of copies was lower (P < 0.01) than that of soybean in pigs fed on diets based on lupin or chickpea. The number of copies of the different bacterial groups in animals fed on the casein-based diet was lower (P < 0.01) than that of soybean for lactobacilli and bacteroides, but was higher than that of soybean for bifidobacteria, enterobacteria and the Escherichia/Shigella group. This information suggests that lupin or chickpea feeding might induce a benefit in the microbiota composition of Iberian pigs in their final productive stages.
