摘要
This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.
This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.
作者
Ammar AlGburi
Anna Volski
Carla Cugini
Emily M. Walsh
Vladimir A. Chistyakov
Maria S. Mazanko
Anzhelica B. Bren
Leon M. T. Dicks
Michael L. Chikindas
Ammar AlGburi;Anna Volski;Carla Cugini;Emily M. Walsh;Vladimir A. Chistyakov;Maria S. Mazanko;Anzhelica B. Bren;Leon M. T. Dicks;Michael L. Chikindas(Department of Biochemistry and Microbiology, Rutgers State University, New Brunswick, NJ, USA;Department of Microbiology, Veterinary College, Diyala University, Baqubah, Iraq;School of Arts and Science, Rutgers State University, New Brunswick, NJ, USA;Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA;Department of Plant Biology and Pathology, Rutgers State University, New Brunswick, NJ, USA;D. I. Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia;Department of Microbiology, University of Stellenbosch, Matieland, South Africa;Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA;Astrabiol, LLC, Highland Park, NJ, USA;Center for Digestive Health, New Jersey Institute for Food, Nutrition and Health, New Brunswick, NJ, USA)
